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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1992-6-16
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pubmed:abstractText |
Myf-5 is a member of a family of muscle-specific transcription factors that activate myogenesis in 10T1/2 fibroblasts. Here we report the analysis of Myf-5 structural domains that are responsible for its biological activity. Site-directed mutagenesis revealed that two clusters of basic amino acids within a conserved basic region and two amphipathic helices within the adjacent HLH domain are essential for sequence-specific DNA binding and hetero-oligomerization, respectively. Transcriptional activation by Myf-5 requires two additional domains located in the amino- and carboxyl-termini. The two domains apparently co-operate since deletion of either one results in inactivation. Chimeric proteins between DNA binding domain of the yeast transcription factor GAL4 and the separate Myf-5 transactivator domains exhibit activity that is enhanced when both regions are combined. Dimerization of Myf-5 with the ubiquitously expressed bHLH protein E12 not only increases the affinity for DNA but also stimulates transactivation independently of DNA binding. The Myf-5 transactivator domains are dependent for activity on a specific amino acid sequence motif within the basic region when Myf-5 activity is mediated through the E-box DNA recognition sequence but not when DNA binding occurs through the GAL4 DNA binding domain. This demonstrates that muscle-specific transactivation by Myf-5 requires the collaboration of two activation domains and the DNA binding region in addition to sequence-specific DNA binding. Transcriptional activation and interaction with DNA are executed by separable domains; however, transactivation is influenced by the basic region in a manner distinguishable from DNA binding.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1648228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1649701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1651276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1652425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1695319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1846022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1846322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1846704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1847137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1903181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-1945842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2099799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2105528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2112746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2114283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2123467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2142255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2155707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2156629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2163343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2205467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2243772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2253873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2300571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2311584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2342565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2350782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2385294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2473006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2479541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2493990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2503252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2509943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2515114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2537150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2546677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2548731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2560751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2571937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2573431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2583111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2721498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2748593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2761538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2761542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2850968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-2965790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-3050531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-3111716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-3175662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-3203380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-3203386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-3416836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-3690668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-3919308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-6185504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-6235151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1582413-6321164
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GAL4 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Myf5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Myogenic Regulatory Factor 5,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1843-55
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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