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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-4-12
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pubmed:abstractText |
A novel chemical-enzymatic approach was developed to facilitate identification of phosphorylation sites in isolated phosphoproteins. ESI-TOF mass spectrometry was used to characterize products from the chemical-enzymatic cleavage of specific phosphorylation sites in bovine alpha-S1 casein and synthetic phosphopeptides containing substitutions at a single phosphorylation site. Further refinements to this approach for identification of protein phosphorylation sites and its utility for the quantification of phosphopeptides by isotope-dilution mass spectrometry are presented.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-10424175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-10572014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11116185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11195502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11283598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11283599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11395414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11476230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11578935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11680868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-11875433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-12007495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-12047170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-12151408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-12440389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-12463352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-12471243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-12923550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-14622963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-14690684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-1499723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-15048979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-15253448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-15253450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-2291484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-3091399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-3106092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-6807135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-7539987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-8059960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-8075429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-8392229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-8811904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-8843139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-9449829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15822919-9819213
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1535-3893
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
424-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15822919-Amino Acid Sequence,
pubmed-meshheading:15822919-Animals,
pubmed-meshheading:15822919-Caseins,
pubmed-meshheading:15822919-Cattle,
pubmed-meshheading:15822919-Molecular Sequence Data,
pubmed-meshheading:15822919-Peptide Fragments,
pubmed-meshheading:15822919-Phosphopeptides,
pubmed-meshheading:15822919-Phosphorylation,
pubmed-meshheading:15822919-Spectrometry, Mass, Electrospray Ionization
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pubmed:articleTitle |
Mapping sites of protein phosphorylation by mass spectrometry utilizing a chemical-enzymatic approach: characterization of products from alpha-S1 casein phosphopeptides.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA. mccormick.daniel@mayo.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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