Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-4-11
pubmed:abstractText
The endothelium synthesizes and releases several vasodilator substances, including vasodilator prostaglandins, NO, and EDHF. NO-mediated relaxations are reduced by various risk factors, such as diabetes mellitus and hypercholesterolemia. However, it remains to be elucidated whether EDHF-mediated relaxations also are reduced by those factors and their combination. In this study, we addressed this point in mice. We used small mesenteric arteries from control, diabetic (streptozotocin-induced), apolipoprotein-E-deficient (ApoE-/-), and diabetic ApoE-/- mice. In control mice, endothelium-dependent relaxations to acetylcholine were largely mediated by EDHF. This EDHF-mediated component was slightly reduced in diabetic mice, preserved in ApoE-/- mice, and markedly reduced in diabetic ApoE-/- mice with an increase in NO-mediated component and a negative contribution of indomethacin-sensitive endothelium-derived contracting factor (EDCF). Endothelium-independent relaxations to sodium nitroprusside or NS1619, a direct opener of calcium-activated K channels, were attenuated in ApoE-/- and diabetic ApoE-/- mice. Endothelium-dependent hyperpolarizations were significantly reduced in diabetic mice, preserved in ApoE-/- mice, and again markedly reduced in diabetic ApoE-/- mice. These results indicate that hypercholesterolemia alone minimally affects the EDHF-mediated relaxations, and diabetes mellitus significantly attenuated the responses, whereas their combination markedly attenuates the responses with a compensatory involvement of NO and a negative contribution of EDCF.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine, http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins E, http://linkedlifedata.com/resource/pubmed/chemical/Benzimidazoles, http://linkedlifedata.com/resource/pubmed/chemical/Biological Factors, http://linkedlifedata.com/resource/pubmed/chemical/Blood Glucose, http://linkedlifedata.com/resource/pubmed/chemical/Endothelium-Dependent Relaxing..., http://linkedlifedata.com/resource/pubmed/chemical/Lipids, http://linkedlifedata.com/resource/pubmed/chemical/NS 1619, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nitroprusside, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents, http://linkedlifedata.com/resource/pubmed/chemical/endothelium-dependent...
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0160-2446
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
485-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15821445-Acetylcholine, pubmed-meshheading:15821445-Animals, pubmed-meshheading:15821445-Apolipoproteins E, pubmed-meshheading:15821445-Benzimidazoles, pubmed-meshheading:15821445-Biological Factors, pubmed-meshheading:15821445-Blood Glucose, pubmed-meshheading:15821445-Diabetes Mellitus, Experimental, pubmed-meshheading:15821445-Dose-Response Relationship, Drug, pubmed-meshheading:15821445-Endothelium, Vascular, pubmed-meshheading:15821445-Endothelium-Dependent Relaxing Factors, pubmed-meshheading:15821445-Hypercholesterolemia, pubmed-meshheading:15821445-Lipids, pubmed-meshheading:15821445-Membrane Potentials, pubmed-meshheading:15821445-Mesenteric Arteries, pubmed-meshheading:15821445-Mice, pubmed-meshheading:15821445-Mice, Inbred C57BL, pubmed-meshheading:15821445-Mice, Knockout, pubmed-meshheading:15821445-Nitric Oxide, pubmed-meshheading:15821445-Nitroprusside, pubmed-meshheading:15821445-Potassium Channels, pubmed-meshheading:15821445-Vasodilation, pubmed-meshheading:15821445-Vasodilator Agents
pubmed:year
2005
pubmed:articleTitle
Influence of diabetes mellitus, hypercholesterolemia, and their combination on EDHF-mediated responses in mice.
pubmed:affiliation
Department of Cardiovascular Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't