Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2005-4-11
pubmed:abstractText
The cultivation of exotic Penaeus vannamei in Thailand began on a very limited scale in the late 1990s, but a Thai government ban on the cultivation of P. monodon in freshwater areas in 2000 led many Thai shrimp farmers to shift to cultivation of P. vannamei. Alarmed by the possibility of Taura syndrome virus (TSV) introduction, the Thai Department of Fisheries required that imported stocks of P. vannamei be certified free of TSV by RT-PCR (Reverse Trasciption Polymerase Chain Reaction) testing. During the interval of allowed importation, over 150,000 broodstock shrimp were imported, 67% of these from China and Taiwan. Despite the safeguards, TSV outbreaks occurred and we confirmed the first outbreak by RT-PCR in early 2003. This resulted in a governmental ban on all shrimp broodstock imports from February 2003, but TSV outbreaks have continued, possibly due to original introductions or to the continued illegal importation of stocks. To determine the origin of the TSV in Thailand, the viral coat protein gene VP1 was amplified by RT-PCR from several shrimp specimens found positive for TSV by RT-PCR from January to November 2003. These included 7 samples from P. vannamei disease outbreaks in Thailand, 3 other non-diseased shrimp samples from Thailand and Burma and 6 samples including P. vannamei and P. japonicus from China. Comparison revealed that the Thai, Burmese and Chinese TSV types formed a clade distinct from a clade of TSV types from the Americas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0177-5103
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-6
pubmed:dateRevised
2006-11-28
pubmed:meshHeading
pubmed-meshheading:15819424-Amino Acid Sequence, pubmed-meshheading:15819424-Animals, pubmed-meshheading:15819424-Aquaculture, pubmed-meshheading:15819424-Base Sequence, pubmed-meshheading:15819424-Cluster Analysis, pubmed-meshheading:15819424-DNA-Binding Proteins, pubmed-meshheading:15819424-Demography, pubmed-meshheading:15819424-Molecular Sequence Data, pubmed-meshheading:15819424-Penaeidae, pubmed-meshheading:15819424-Phylogeny, pubmed-meshheading:15819424-Picornaviridae, pubmed-meshheading:15819424-Plant Proteins, pubmed-meshheading:15819424-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15819424-Sequence Alignment, pubmed-meshheading:15819424-Sequence Analysis, DNA, pubmed-meshheading:15819424-Thailand, pubmed-meshheading:15819424-Trans-Activators, pubmed-meshheading:15819424-Transcription Factors
pubmed:year
2005
pubmed:articleTitle
Taura syndrome virus (TSV) in Thailand and its relationship to TSV in China and the Americas.
pubmed:affiliation
Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't