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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-5-18
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pubmed:abstractText |
Treatment of Jurkat cells with specific inhibitors of protein kinase CK2 induces apoptosis. Here we provide evidence that the anti-apoptotic effect of CK2 can be at least partially mediated by upregulation of the Akt/PKB pathway. Such a conclusion is based on the following observations: (1) inhibition of CK2 by cell treatment with two structurally unrelated CK2 inhibitors induces downregulation of Akt/PKB, as judged from decreased phosphorylation of its physiological targets, and immunoprecipitate kinase assay; (2) similar results are observed upon reduction of CK2 catalytic subunit by the RNA-interference technique; (3) Akt/PKB Ser129 is phosphorylated by CK2 in vitro and in vivo; (4) such a phosphorylation of activated Akt/PKB correlates with a further increase in catalytic activity. These data disclose an unanticipated mechanism by which constitutive phosphorylation by CK2 may be required for maximal activation of Akt/PKB.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1350-9047
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
668-77
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15818404-Amino Acid Sequence,
pubmed-meshheading:15818404-Casein Kinase II,
pubmed-meshheading:15818404-Catalytic Domain,
pubmed-meshheading:15818404-Cell Line,
pubmed-meshheading:15818404-Down-Regulation,
pubmed-meshheading:15818404-Enzyme Induction,
pubmed-meshheading:15818404-Humans,
pubmed-meshheading:15818404-Molecular Sequence Data,
pubmed-meshheading:15818404-Mutation,
pubmed-meshheading:15818404-Phosphorylation,
pubmed-meshheading:15818404-Phosphoserine,
pubmed-meshheading:15818404-Protein Kinase Inhibitors,
pubmed-meshheading:15818404-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15818404-Proto-Oncogene Proteins,
pubmed-meshheading:15818404-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:15818404-RNA Interference,
pubmed-meshheading:15818404-Signal Transduction,
pubmed-meshheading:15818404-Up-Regulation
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pubmed:year |
2005
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pubmed:articleTitle |
Protein kinase CK2 phosphorylates and upregulates Akt/PKB.
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pubmed:affiliation |
Venetian Institute of Molecular Medicine (VIMM), University of Padova, Padova, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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