Linked Life Data

15817759

Source:http://linkedlifedata.com/resource/pubmed/id/15817759

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-4-8
pubmed:abstractText
Under current practices of mouse colony maintenance, sera from mice are analyzed for antibodies against several widespread infectious pathogens by conventional immunoassays, generally enzyme-linked immunosorbent assay (ELISA). To test for multiple agents, these methods consume large volumes of mouse serum and are laborious and time-consuming. More efficient immunoassays, using small amounts of sample, are therefore needed. Accordingly, we have developed a novel multiplex diagnostic system that employs fluorescent microbeads, coated with purified antigens, for simultaneous serodetection of 10 mouse infectious agents. Individually identifiable, fluorescent microbeads were coated with antigens from Sendai virus, mouse hepatitis virus, Theiler's mouse encephalomyelitis virus/GDVII strain, mouse minute virus, mouse cytomegalovirus, respiratory enteric orphan virus (Reo-3 virus), mouse parvovirus, calf rotavirus for epizootic diarrhea virus of infant mice, vaccinia virus for ectromelia virus, and Mycoplasma pulmonis. Standard sera, singly positive for antibodies to individual infectious agents, were generated by inoculation of BALB/cj and C57BL/6j mice. Sera from these experimentally infected mice, as well as sera from naturally infected mice, were analyzed using a mixture of microbeads coated with antigens of the 10 infectious agents listed above. Results demonstrated that the multiplex assay was at least as sensitive and specific as ELISA for serodetection. Importantly, the multiplex assay required only 1 microliter of serum for simultaneous serodetection of the 10 mouse infectious agents in one reaction vessel. Thus, this multiplex microbead assay is a reliable, efficient, and cost-effective diagnostic modality that will impact serosurveillance of mice used in research.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-11777827, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-11939734, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-12093688, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-12204954, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-12522048, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-15154612, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-15357318, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-15370458, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-2267938, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-3011855, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-3084866, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-6298492, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-8523521, http://linkedlifedata.com/resource/pubmed/commentcorrection/15817759-9773885
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
513-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Simultaneous serodetection of 10 highly prevalent mouse infectious pathogens in a single reaction by multiplex analysis.
pubmed:affiliation
Center for Comparative Medicine, and Department of Pathology and Laboratory Medicine, University of California at Davis, Davis, California 95616, USA. ihkhan@ucdavis.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural