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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-7-4
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pubmed:abstractText |
We developed a novel in vivo multiple myeloma (MM) model by engrafting the interleukin 6 (IL-6)-dependent human MM cell line INA-6 into severe combined immunodeficiency (SCID) mice previously given implants of a human fetal bone chip (SCID-hu mice). INA-6 cells require either exogenous human IL-6 (huIL-6) or bone marrow stromal cells (BMSCs) to proliferate in vitro. In this model, we monitored the in vivo growth of INA-6 cells stably transduced with a green fluorescent protein (GFP) gene (INA-6GFP+ cells). INA-6 MM cells engrafted in SCID-hu mice but not in SCID mice that had not been given implants of human fetal bone. The level of soluble human IL-6 receptor (shuIL-6R) in murine serum and fluorescence imaging of host animals were sensitive indicators of tumor growth. Dexamethasone as well as experimental drugs, such as Atiprimod and B-B4-DM1, were used to confirm the utility of the model for evaluation of anti-MM agents. We report that this model is highly reproducible and allows for evaluation of investigational drugs targeting IL-6-dependent MM cells in the human bone marrow (huBM) milieu.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-11477577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-11486317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-11920233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-12459731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-12548578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-14684154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-15190512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-15292058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-1558966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-15705788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-2971269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-7520766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-7522631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-7680919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-8104540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-8443818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-8608240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-8822916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-9226176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-9310495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-9763577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15817674-9811723
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-4971
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pubmed:author |
pubmed-author:AndersonKenneth CKC,
pubmed-author:BurgerRenateR,
pubmed-author:CarrascoDaniel RDR,
pubmed-author:CatleyLaurenceL,
pubmed-author:FramRobertR,
pubmed-author:GoldmacherVictor SVS,
pubmed-author:JacobGary SGS,
pubmed-author:MunshiNikhil CNC,
pubmed-author:MunshiViditV,
pubmed-author:NeriPaolaP,
pubmed-author:ShammasMasood AMA,
pubmed-author:TassonePierfrancescoP,
pubmed-author:VenutaSalvatoreS
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
713-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15817674-Animals,
pubmed-meshheading:15817674-Bone Transplantation,
pubmed-meshheading:15817674-Cell Line, Tumor,
pubmed-meshheading:15817674-Dexamethasone,
pubmed-meshheading:15817674-Disease Models, Animal,
pubmed-meshheading:15817674-Fetal Tissue Transplantation,
pubmed-meshheading:15817674-Green Fluorescent Proteins,
pubmed-meshheading:15817674-Humans,
pubmed-meshheading:15817674-Immunotoxins,
pubmed-meshheading:15817674-Mice,
pubmed-meshheading:15817674-Mice, SCID,
pubmed-meshheading:15817674-Multiple Myeloma,
pubmed-meshheading:15817674-Neoplasm Transplantation,
pubmed-meshheading:15817674-Receptors, Interleukin-6,
pubmed-meshheading:15817674-Recombinant Proteins,
pubmed-meshheading:15817674-Spiro Compounds,
pubmed-meshheading:15817674-Transduction, Genetic,
pubmed-meshheading:15817674-Transplantation, Heterologous
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pubmed:year |
2005
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pubmed:articleTitle |
A clinically relevant SCID-hu in vivo model of human multiple myeloma.
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pubmed:affiliation |
Jerome Lipper Multiple Myeloma Center, Dana-Farber Cancer Institute and VA Boston Healthcare System, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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