Linked Life Data

15815918

Source:http://linkedlifedata.com/resource/pubmed/id/15815918

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-7-29
pubmed:abstractText
Several Arabidopsis mutants of the ecotype Dijon were isolated that show resistance to the herbicide acifluorfen, which inactivates protoporphyrinogen oxidase (PPOX), an enzyme of tetrapyrrole biosynthesis. This enzyme provides protoporphyrin for both Mg chelatase and ferrochelatase at the branchpoint, which leads to chlorophyll and heme, respectively. One of the mutations, aci5-3, displays semidominant inheritance. Heterozygous progeny showed yellow-green leaves, while the homozygous seedlings were white and inviable, but could be rescued by supplementing the medium with sugar. Interestingly, the expression of neither of the two forms of PPOX was altered in the mutant, but the rate of synthesis of 5-aminolevulinate, the precursor of all tetrapyrroles, was drastically reduced. Genetic mapping revealed the mutant locus is closely linked to the ch42 marker, which is itself located in the CHLI-1 gene which codes for one of the three subunits of Mg chelatase. The cs mutant also shows a defect in this gene, and test for allelism with aci5-3 confirmed that the two mutations are allelic. Sequencing of the wild type and aci5-3 alleles of CHLI-1 revealed a single base change (G718A), which results in a D240N substitution in the CHLI-1 protein. In the homozygous aci5-3 mutant no CHLI-1 RNA or protein could be detected. Strikingly, CHLH and CHLI-2 transcripts were also absent. This indicates the existence of a feedback-regulatory mechanism that inactivates the genes encoding certain Mg chelatase subunits. The basis for the semidominant inheritance pattern and the relationship between herbicide resistance and modified gene expression is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1617-4615
pubmed:author
pubmed:issnType
Print
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
311-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15815918-Aminolevulinic Acid, pubmed-meshheading:15815918-Arabidopsis, pubmed-meshheading:15815918-Base Sequence, pubmed-meshheading:15815918-Blotting, Northern, pubmed-meshheading:15815918-Blotting, Western, pubmed-meshheading:15815918-Chromosome Mapping, pubmed-meshheading:15815918-DNA Primers, pubmed-meshheading:15815918-Drug Resistance, pubmed-meshheading:15815918-Gene Expression Regulation, Plant, pubmed-meshheading:15815918-Herbicides, pubmed-meshheading:15815918-Inheritance Patterns, pubmed-meshheading:15815918-Lyases, pubmed-meshheading:15815918-Molecular Sequence Data, pubmed-meshheading:15815918-Mutation, pubmed-meshheading:15815918-Nitrobenzoates, pubmed-meshheading:15815918-Oxidoreductases Acting on CH-CH Group Donors, pubmed-meshheading:15815918-Pigmentation, pubmed-meshheading:15815918-Protein Subunits, pubmed-meshheading:15815918-Protoporphyrinogen Oxidase, pubmed-meshheading:15815918-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15815918-Sequence Analysis, DNA, pubmed-meshheading:15815918-Tetrapyrroles
pubmed:year
2005
pubmed:articleTitle
An Arabidopsis mutant that is resistant to the protoporphyrinogen oxidase inhibitor acifluorfen shows regulatory changes in tetrapyrrole biosynthesis.
pubmed:affiliation
Department of Genetics, Faculty of Biology, M.V. Lomonosov State University, Leninskie Gori 1, Building 12, 119899, Moscow, Russia.
pubmed:publicationType
Journal Article, Comparative Study