|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1992-6-18
|
|
pubmed:abstractText |
The (+)-enantiomer of 7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene (BP-7,8-diol) is a diagnostic probe for cytochrome P-450 and non-cytochrome P-450 pathways of dihydrodiol epoxidation. The principle products of epoxidation are the (+)-syn-dihydrodiol epoxide [(+)-syn-BPDE] and the (-)-anti-dihydrodiol epoxide [(-)-anti-BPDE]. Chromatographic conditions are described that separate the major deoxynucleoside 3',5'-bisphosphate adducts derived from these dihydrodiol epoxides on commercial poly(ethylenimine) thin-layer plates. Inclusion of boric acid and magnesium chloride in the D4 solvent is a key feature of the separation. Reasonable separation of these bisphosphate adducts from the major deoxynucleoside 3',5'-bisphosphate adduct derived from (+)-anti-BPDE is also observed. 32P-Postlabeling analysis of DNA adducts produced following topical administration of benzo[a]pyrene to mouse skin suggests that cytochrome P-450 plays a major role in its metabolism to DNA binding derivatives.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/7,8-Dihydro-7,8-dihydroxybenzo(a)pyr...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Benzo(a)pyrene,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Adducts,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydroxydihydrobenzopyrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorus Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/benzo(a)pyrene 7,8-dihydrodiol,
http://linkedlifedata.com/resource/pubmed/chemical/benzo(a)pyrene-7,8-dihydrodiol-9,10-...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0893-228X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
5
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
19-25
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:1581531-7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide,
pubmed-meshheading:1581531-Adenosine Triphosphate,
pubmed-meshheading:1581531-Animals,
pubmed-meshheading:1581531-Autoradiography,
pubmed-meshheading:1581531-Benzo(a)pyrene,
pubmed-meshheading:1581531-Chromatography, Thin Layer,
pubmed-meshheading:1581531-DNA,
pubmed-meshheading:1581531-DNA Adducts,
pubmed-meshheading:1581531-Dihydroxydihydrobenzopyrenes,
pubmed-meshheading:1581531-Female,
pubmed-meshheading:1581531-Isotope Labeling,
pubmed-meshheading:1581531-Male,
pubmed-meshheading:1581531-Mice,
pubmed-meshheading:1581531-Mice, Inbred Strains,
pubmed-meshheading:1581531-Nucleotides,
pubmed-meshheading:1581531-Phosphorus Radioisotopes,
pubmed-meshheading:1581531-Rats,
pubmed-meshheading:1581531-Rats, Inbred Strains,
pubmed-meshheading:1581531-Stereoisomerism
|
|
pubmed:articleTitle |
Separation of (+)-syn- and (-)-anti-benzo[a]pyrene dihydrodiol epoxide-DNA adducts in 32P-postlabeling analysis.
|
|
pubmed:affiliation |
Department of Chemistry, Wayne State University, Detroit, Michigan 48202.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
