Linked Life Data

15814967

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pubmed-article:15814967pubmed:abstractTextThe LIAISON system is a fully automated system based on chemiluminescence and antigen bound to magnetic microparticles. The system allows fast and precise measurement of Toxoplasma-specific immunoglobulin G (IgG) and IgM antibody levels and measurement of the IgG avidity index even at low levels of Toxoplasma-specific IgG antibodies in a single step without manual interference. Seven European centers participated in a multicenter evaluation of the LIAISON system. The sensitivity and specificity of the LIAISON system compared to the Sabin-Feldman dye test were 99.3 and 96.8%, respectively. In a comparison of the LIAISON Toxoplasma-specific IgM assay with an immunosorbent agglutination assay, the LIAISON assay had a sensitivity of 96.7% and a specificity of 95.4%. The LIAISON IgG assay showed agreements of 91, 100, and 100% with the AXSYM IgG (Abbott), VIDAS IgG (bioMerieux), and Platelia IgG (Bio-Rad) assays, respectively. The LIAISON IgM assay showed agreements of 95% with the AXSYM IgM and Platelia IgM assays, 96% with the ISAGA IgM assay (bioMerieux), and 97% with the VIDAS IgM assay. The coefficient of correlation between the LIAISON system and the VIDAS Toxoplasma-specific IgG avidity index was 0.81. By use of the Toxoplasma-specific IgG avidity index assay with specific IgM-positive samples, the diagnosis of infection with Toxoplasma gondii in early pregnancy has been improved significantly. The LIAISON avidity assay is a valuable assay for the exclusion of recently acquired infection with T. gondii (less than 4 months) in pregnant women, and it decreases significantly the necessity for follow-up testing.lld:pubmed
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pubmed-article:15814967pubmed:dateRevised2010-9-20lld:pubmed
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pubmed-article:15814967pubmed:articleTitleEuropean multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index.lld:pubmed
pubmed-article:15814967pubmed:affiliationWHO/FAO International Centre for Research and Reference on Toxoplasmosis, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. eskildp@dadlnet.dklld:pubmed
pubmed-article:15814967pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15814967pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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