Linked Life Data

15814791

Source:http://linkedlifedata.com/resource/pubmed/id/15814791

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2005-4-7
pubmed:abstractText
We investigated how spontaneous activity is generated in developing, hyperexcitable networks. We focused our study on the embryonic chick spinal cord, a preparation that exhibits rhythmic discharge on multiple timescales: slow episodes (lasting minutes) and faster intraepisode cycling (approximately 1 Hz frequency). For this purpose, we developed a mean field model of a recurrent network with slow chloride dynamics and a fast depression variable. We showed that the model, in addition to providing a biophysical mechanism for the slow dynamics, was able to account for the experimentally observed activity. The model made predictions on how interval and duration of episodes are affected when changing chloride-mediated synaptic transmission or chloride flux across cell membrane. These predictions guided experiments, and the model results were compared with experimental data obtained with electrophysiological recordings. We found agreement when transmission was affected through changes in synaptic conductance and good qualitative agreement when chloride flux was varied through changes in external chloride concentration or in the rate of the Na+-K+-2Cl- cotransporter. Furthermore, the model made predictions about the time course of intracellular chloride concentration and chloride reversal potential and how these are affected by changes in synaptic conductance. Based on the comparison between modeling and experimental results, we propose that chloride dynamics could be an important mechanism in rhythm generation in the developing chick spinal cord.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
6
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3601-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15814791-2-Amino-5-phosphonovalerate, pubmed-meshheading:15814791-6-Cyano-7-nitroquinoxaline-2,3-dione, pubmed-meshheading:15814791-Action Potentials, pubmed-meshheading:15814791-Animals, pubmed-meshheading:15814791-Bicuculline, pubmed-meshheading:15814791-Bumetanide, pubmed-meshheading:15814791-Chick Embryo, pubmed-meshheading:15814791-Chlorides, pubmed-meshheading:15814791-Drug Interactions, pubmed-meshheading:15814791-Excitatory Amino Acid Antagonists, pubmed-meshheading:15814791-GABA Antagonists, pubmed-meshheading:15814791-Intracellular Space, pubmed-meshheading:15814791-Neural Inhibition, pubmed-meshheading:15814791-Neural Networks (Computer), pubmed-meshheading:15814791-Neurons, pubmed-meshheading:15814791-Nonlinear Dynamics, pubmed-meshheading:15814791-Patch-Clamp Techniques, pubmed-meshheading:15814791-Periodicity, pubmed-meshheading:15814791-Sodium Potassium Chloride Symporter Inhibitors, pubmed-meshheading:15814791-Spinal Cord, pubmed-meshheading:15814791-Synaptic Transmission, pubmed-meshheading:15814791-Time Factors
pubmed:year
2005
pubmed:articleTitle
Modeling spontaneous activity in the developing spinal cord using activity-dependent variations of intracellular chloride.
pubmed:affiliation
Laboratory of Neural Control, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.