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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2005-4-7
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pubmed:abstractText |
The cytotoxic function of CD178 (Fas ligand (FasL)) is critical to the maintenance of peripheral tolerance and immune-mediated tissue pathology. The active site of FasL resides at the FasL extracellular region (FasL(Ext)) and it functions through binding/cross-linking Fas receptor on target cells. In this study, we report that FasL(Ext)-mediated cytotoxicity is regulated by the FasL cytoplasmic tail (FasL(Cyt)). Deleting the N-terminal 2-70 aa (delta70) or N-terminal 2-33 aa (delta33) reduced the cytotoxic strength as much as 30- to 100-fold. By contrast, change in the cytotoxic strength was not observed with FasL deleted of the proline-rich domains (45-74 aa, delta PRD) in the FasL(Cyt). Our study identifies a novel function of FasL(Cyt) and demonstrates that FasL(2-33), a sequence unique to FasL, is critically required for the optimal expression of FasL(Ext)-mediated cytotoxicity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4470-4
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15814666-Amino Acid Sequence,
pubmed-meshheading:15814666-Animals,
pubmed-meshheading:15814666-Base Sequence,
pubmed-meshheading:15814666-COS Cells,
pubmed-meshheading:15814666-Cell Line,
pubmed-meshheading:15814666-Cell Line, Tumor,
pubmed-meshheading:15814666-Cytoplasm,
pubmed-meshheading:15814666-Cytotoxicity, Immunologic,
pubmed-meshheading:15814666-DNA, Complementary,
pubmed-meshheading:15814666-Fas Ligand Protein,
pubmed-meshheading:15814666-Humans,
pubmed-meshheading:15814666-Jurkat Cells,
pubmed-meshheading:15814666-Membrane Glycoproteins,
pubmed-meshheading:15814666-Mice,
pubmed-meshheading:15814666-Molecular Sequence Data,
pubmed-meshheading:15814666-NIH 3T3 Cells,
pubmed-meshheading:15814666-Peptide Fragments,
pubmed-meshheading:15814666-Recombinant Proteins,
pubmed-meshheading:15814666-Sequence Deletion
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pubmed:year |
2005
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pubmed:articleTitle |
Fas ligand (CD178) cytoplasmic tail is a positive regulator of Fas ligand-mediated cytotoxicity.
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pubmed:affiliation |
Department of Medicine II, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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