Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-4-5
pubmed:abstractText
The tumor suppressor retinoblastoma gene (RB) is a well-known regulator of cell cycle progression, differentiation and apoptosis. Inactivation of this gene is closely associated with the development of many human malignancies. Previous studies of testicular germ cell tumors (TGCTs) revealed lower levels of RB mRNA and protein compared with normal testis tissue. In this study, we examined the mechanism behind the reduced expression of RB mRNA and protein in TGCTs. The mRNA and protein expression were down-regulated in JKT-1 human testicular seminoma cells, compared with other cell lines. Promoter activity from a construct without a hGABP/E4TF1-binding site was markedly reduced in A549 human lung adenocarcinoma cells, but not in JKT-1. Furthermore, promoter activity from a plasmid carrying a point mutation at the hGABP/E4TF1 site was reduced in A549 cells, but not altered in JKT-1 cells. These differential responses of RB promoter constructs raise the possibility that the hGABP/E4TF1 site does not act as a positive regulatory element in JKT-1 cells, and the RB gene might be down-regulated at the transcriptional level in JKT-1 cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1021-335X
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
871-4
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15809752-Adenocarcinoma, pubmed-meshheading:15809752-Base Sequence, pubmed-meshheading:15809752-Cell Line, Tumor, pubmed-meshheading:15809752-DNA Primers, pubmed-meshheading:15809752-DNA-Binding Proteins, pubmed-meshheading:15809752-GA-Binding Protein Transcription Factor, pubmed-meshheading:15809752-Genes, Reporter, pubmed-meshheading:15809752-Genes, Retinoblastoma, pubmed-meshheading:15809752-Humans, pubmed-meshheading:15809752-Luciferases, pubmed-meshheading:15809752-Lung Neoplasms, pubmed-meshheading:15809752-Male, pubmed-meshheading:15809752-Plasmids, pubmed-meshheading:15809752-Point Mutation, pubmed-meshheading:15809752-Promoter Regions, Genetic, pubmed-meshheading:15809752-RNA, Messenger, pubmed-meshheading:15809752-Seminoma, pubmed-meshheading:15809752-Testicular Neoplasms, pubmed-meshheading:15809752-Transcription, Genetic, pubmed-meshheading:15809752-Transcription Factors, pubmed-meshheading:15809752-Transfection
pubmed:year
2005
pubmed:articleTitle
Differential response at the hGABP/E4TF1 site of retinoblastoma gene promoter in human testicular seminoma cells.
pubmed:affiliation
Department of Molecular-Targeting Cancer Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajiicho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't