| pubmed-article:15809708 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15809708 | lifeskim:mentions | umls-concept:C0242957 | lld:lifeskim |
| pubmed-article:15809708 | lifeskim:mentions | umls-concept:C0555198 | lld:lifeskim |
| pubmed-article:15809708 | lifeskim:mentions | umls-concept:C0012920 | lld:lifeskim |
| pubmed-article:15809708 | lifeskim:mentions | umls-concept:C1704824 | lld:lifeskim |
| pubmed-article:15809708 | lifeskim:mentions | umls-concept:C0178655 | lld:lifeskim |
| pubmed-article:15809708 | lifeskim:mentions | umls-concept:C1521725 | lld:lifeskim |
| pubmed-article:15809708 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:15809708 | pubmed:dateCreated | 2005-4-5 | lld:pubmed |
| pubmed-article:15809708 | pubmed:abstractText | Pediatric malignant non-brainstem glioma (PMNBG) is a rare tumor that accounts for only about five percent of childhood intracranial neoplasms. DNA topoisomerase IIalpha (TIIalpha) is a novel marker of cell-cycle turnover and a target of high-risk chemotherapy in PMNBG. We have shown that TIIalpha protein expression strongly correlates with event-free and overall survival in these malignancies. The molecular mechanism causing the varying TIIalpha protein expression in PMNBG remains unknown. Utilizing a combined approach of immunocytochemistry-based morphology guidance, laser-assisted microdissection and quantitative real-time PCR, we report a low-level co-amplification of the neighboring TIIalpha and Her-2/neu gene loci on chromosome 17q11-q22 in one of seventeen examined PMNBGs. Analysis of both genes by real-time PCR in the crude tumor samples without prior tissue heterogeneity reduction via laser microdissection, resulted in loss of detection of amplification of the syngeneic Her-2/neu locus. Gene dosage assessment in a microscopically distant tumor area revealed no amplification of either gene. Our results suggest that low-level amplification of the TIIalpha gene locus may be a sporadic mechanism of increased TIIalpha protein expression in PMNBG, which can coincide with low-level amplification of Her-2/neu. The observed intratumor genetic heterogeneity for TIIalpha in PMNBGs may have an impact on the relevance of TIIalpha as a biological constituent of outcome in these neoplasms. | lld:pubmed |
| pubmed-article:15809708 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15809708 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15809708 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15809708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15809708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15809708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15809708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15809708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15809708 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15809708 | pubmed:month | May | lld:pubmed |
| pubmed-article:15809708 | pubmed:issn | 1019-6439 | lld:pubmed |
| pubmed-article:15809708 | pubmed:author | pubmed-author:HamiltonRonal... | lld:pubmed |
| pubmed-article:15809708 | pubmed:author | pubmed-author:PollackIan... | lld:pubmed |
| pubmed-article:15809708 | pubmed:author | pubmed-author:WernerMartinM | lld:pubmed |
| pubmed-article:15809708 | pubmed:author | pubmed-author:BredelMarkusM | lld:pubmed |
| pubmed-article:15809708 | pubmed:author | pubmed-author:VolkBenediktB | lld:pubmed |
| pubmed-article:15809708 | pubmed:author | pubmed-author:LassmannSilke... | lld:pubmed |
| pubmed-article:15809708 | pubmed:author | pubmed-author:KnothRolfR | lld:pubmed |
| pubmed-article:15809708 | pubmed:author | pubmed-author:BredelClaudia... | lld:pubmed |
| pubmed-article:15809708 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15809708 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:15809708 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15809708 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15809708 | pubmed:pagination | 1187-92 | lld:pubmed |
| pubmed-article:15809708 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:15809708 | pubmed:meshHeading | pubmed-meshheading:15809708... | lld:pubmed |
| pubmed-article:15809708 | pubmed:meshHeading | pubmed-meshheading:15809708... | lld:pubmed |
| pubmed-article:15809708 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15809708 | pubmed:articleTitle | DNA topoisomerase IIalpha and Her-2/neu gene dosages in pediatric malignant gliomas. | lld:pubmed |
| pubmed-article:15809708 | pubmed:affiliation | Department of General Neurosurgery, Neurocenter, University of Freiburg, D-79106 Freiburg, Germany. mbredel@stanford.edu | lld:pubmed |
| pubmed-article:15809708 | pubmed:publicationType | Journal Article | lld:pubmed |
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