Source:http://linkedlifedata.com/resource/pubmed/id/15809708
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2005-4-5
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pubmed:abstractText |
Pediatric malignant non-brainstem glioma (PMNBG) is a rare tumor that accounts for only about five percent of childhood intracranial neoplasms. DNA topoisomerase IIalpha (TIIalpha) is a novel marker of cell-cycle turnover and a target of high-risk chemotherapy in PMNBG. We have shown that TIIalpha protein expression strongly correlates with event-free and overall survival in these malignancies. The molecular mechanism causing the varying TIIalpha protein expression in PMNBG remains unknown. Utilizing a combined approach of immunocytochemistry-based morphology guidance, laser-assisted microdissection and quantitative real-time PCR, we report a low-level co-amplification of the neighboring TIIalpha and Her-2/neu gene loci on chromosome 17q11-q22 in one of seventeen examined PMNBGs. Analysis of both genes by real-time PCR in the crude tumor samples without prior tissue heterogeneity reduction via laser microdissection, resulted in loss of detection of amplification of the syngeneic Her-2/neu locus. Gene dosage assessment in a microscopically distant tumor area revealed no amplification of either gene. Our results suggest that low-level amplification of the TIIalpha gene locus may be a sporadic mechanism of increased TIIalpha protein expression in PMNBG, which can coincide with low-level amplification of Her-2/neu. The observed intratumor genetic heterogeneity for TIIalpha in PMNBGs may have an impact on the relevance of TIIalpha as a biological constituent of outcome in these neoplasms.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Topoisomerases, Type II,
http://linkedlifedata.com/resource/pubmed/chemical/DNA topoisomerase II alpha,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-2
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1019-6439
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1187-92
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15809708-Antigens, Neoplasm,
pubmed-meshheading:15809708-Brain Neoplasms,
pubmed-meshheading:15809708-Child,
pubmed-meshheading:15809708-Chromosomes, Human, Pair 17,
pubmed-meshheading:15809708-DNA Topoisomerases, Type II,
pubmed-meshheading:15809708-DNA-Binding Proteins,
pubmed-meshheading:15809708-Gene Amplification,
pubmed-meshheading:15809708-Gene Dosage,
pubmed-meshheading:15809708-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:15809708-Glioma,
pubmed-meshheading:15809708-Humans,
pubmed-meshheading:15809708-Immunohistochemistry,
pubmed-meshheading:15809708-Polymerase Chain Reaction,
pubmed-meshheading:15809708-Receptor, erbB-2
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pubmed:year |
2005
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pubmed:articleTitle |
DNA topoisomerase IIalpha and Her-2/neu gene dosages in pediatric malignant gliomas.
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pubmed:affiliation |
Department of General Neurosurgery, Neurocenter, University of Freiburg, D-79106 Freiburg, Germany. mbredel@stanford.edu
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pubmed:publicationType |
Journal Article
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