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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2005-4-5
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pubmed:abstractText |
We have previously reported that the intact PTH molecule (1-84) stimulates proliferation of human umbilical vein endothelial cells (HUVECs). To define the bioactive portion of the PTH molecule we utilized amino, mid and carboxy-terminal PTH fragments. Carboxy- but not amino-terminal fragments were equivalent to the intact PTH molecule in stimulating [3H]thymidine incorporation in HUVEC. Carboxy- but not amino-terminal PTH fragments increased intracellular calcium. Blocking the rise in intracellular calcium with calcium chelators abolished PTHs proliferative effect on HUVEC. In contrast to PTH 1-84, the carboxy-terminal fragment effect on [3H]thymidine incorporation was blocked by KN-93 an inhibitor of CaM kinase II. Taken together, these data suggest that the carboxy-terminal PTH is (or contains) the bioactive fragment responsible for the changes in intracellular calcium and thymidine incorporation in HUVEC stimulated with the intact PTH molecule.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0196-9781
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
853-62
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15808916-Benzylamines,
pubmed-meshheading:15808916-Calcium,
pubmed-meshheading:15808916-Calcium-Calmodulin-Dependent Protein Kinase Type 2,
pubmed-meshheading:15808916-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:15808916-Cell Proliferation,
pubmed-meshheading:15808916-Endothelium, Vascular,
pubmed-meshheading:15808916-Gene Expression Profiling,
pubmed-meshheading:15808916-Humans,
pubmed-meshheading:15808916-Parathyroid Hormone,
pubmed-meshheading:15808916-Peptide Fragments,
pubmed-meshheading:15808916-Protein Kinase Inhibitors,
pubmed-meshheading:15808916-Sulfonamides,
pubmed-meshheading:15808916-Thymidine
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pubmed:year |
2005
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pubmed:articleTitle |
Carboxy-terminal PTH fragments stimulate [3H]thymidine incorporation in vascular endothelial cells.
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pubmed:affiliation |
Institute of Molecular Medicine and Genetics, Department of Medicine, Medical College of Georgia, CB-2803, 1120, 15th Street, Augusta, GA 30912, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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