Linked Life Data

15808628

Source:http://linkedlifedata.com/resource/pubmed/id/15808628

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-4-5
pubmed:abstractText
Chronic graft dysfunction is the greatest barrier to long-term graft survival, although the immediate outcome in organ transplantation has been greatly improved. Graft arteriosclerosis is a prominent feature of chronic graft dysfunction. Recipient progenitor cells have been shown to participate in neointimal development in graft arteriosclerosis. The present study investigated the role of recipient endothelial cells in the repair and remodeling after a cold preservation injury in an orthotopic cross-sex abdominal aortic allotransplantation model, namely female Wistar to male Sprague-Dawley rats. Grafts were preserved for 48 hours in 4 degrees C University of Wisconsin (UW) solution for a prolonged cold ischemia (PCI) group or preserved for <1 hour in the control group; or for <1 hour in the presence of feeding with cyclosporine (CyA). A direct in situ polymerase chain reaction (ISPCR) for the SRY gene showed SRY-marked endothelial and smooth muscle-like cells in neointima at 2 weeks in the PCI group, at 4 weeks in the control group, and rarely at 3 months in the CyA group. Staining by H&E showed the aortic graft intima to be thicker in the PCI than in the control group at 4 weeks, but thinning thereafter. The SRY-positive cells correlated with intimal thickness in the PCI and the control group (r = .801 and .825; P < .05 and <.05, respectively), but not in the CyA group (r = .247, P > .5). Our data suggest that prolonged cold preservation promotes recipient cell participation in graft arteriosclerosis after endothelium injury. The early neointimal formation via recipient cells incorporated into arteriosclerotic neointima may delay later intimal thickening. In the aortic allotransplantation model, prolonged cold ischemia may be beneficial for long-term graft survival due to early endothelial replacement. We hypothesize that controlled injury to the graft may serve as a new strategy for treatment of intimal thickening.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0041-1345
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
312-5
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15808628-Animals, pubmed-meshheading:15808628-Aorta, Abdominal, pubmed-meshheading:15808628-Arteriosclerosis, pubmed-meshheading:15808628-Cold Temperature, pubmed-meshheading:15808628-DNA-Binding Proteins, pubmed-meshheading:15808628-Disease Models, Animal, pubmed-meshheading:15808628-Female, pubmed-meshheading:15808628-Male, pubmed-meshheading:15808628-Nuclear Proteins, pubmed-meshheading:15808628-Organ Preservation, pubmed-meshheading:15808628-Polymerase Chain Reaction, pubmed-meshheading:15808628-Rats, pubmed-meshheading:15808628-Rats, Sprague-Dawley, pubmed-meshheading:15808628-Rats, Wistar, pubmed-meshheading:15808628-Sex-Determining Region Y Protein, pubmed-meshheading:15808628-Stem Cell Transplantation, pubmed-meshheading:15808628-Transcription Factors, pubmed-meshheading:15808628-Transplantation, Homologous, pubmed-meshheading:15808628-Transplantation Chimera, pubmed-meshheading:15808628-Tunica Intima
pubmed:articleTitle
Prolonged cold preservation promotes the recipient's cell participating in neointima formation but delays the later graft arteriosclerosis in rat model.
pubmed:affiliation
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't