Source:http://linkedlifedata.com/resource/pubmed/id/15807308
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2005-4-5
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| pubmed:abstractText |
A suitable model analyzing the behavior of well-differentiated squamous cell carcinoma has not yet been established. We tried to establish such a system using a reconstructed oral mucosa, in which T3M-1 squamous cell carcinoma cells were cultured on 3T3 fibroblast-containing collagen gel. Fibroblasts promoted the stratification and keratinization of T3M-1 cells. During growth, the Ki-67 index of T3M-1 cells with fibroblasts was higher than that of T3M-1 cells alone. Fibroblasts increased the expression of involucrin, a differentiating marker of keratinocytes, in T3M-1 cells. They also promoted the invasion of T3M-1 cells into the gel. When T3M-1 cells alone were cultured in a fibroblast-conditioned (FC) medium, the fibroblast-induced phenomena mentioned above were almost replicated. In addition, epidermal growth factqr (EGF) promoted T3M-1 cells growth, but not the invasion. cDNA microarray analysis showed that FC medium increased the expression of EGF receptor and several other mRNAs of T3M-1 cells. The data suggest that T3M-1 cells, under cancer-stromal fibroblast interaction, undergo invasive growth with their well-differentiated squamous phenotype, and that this interaction may be mediated partly by soluble molecules (e.g., EGF) in an autocrine or paracrine pathway. Our system will probably provide a useful model for analyzing the biological behavior of well-differentiated squamous cell carcinoma.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Gels,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Ki-67 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/involucrin
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0344-0338
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
201
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
27-35
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| pubmed:meshHeading |
pubmed-meshheading:15807308-3T3 Cells,
pubmed-meshheading:15807308-Animals,
pubmed-meshheading:15807308-Carcinoma, Squamous Cell,
pubmed-meshheading:15807308-Cell Differentiation,
pubmed-meshheading:15807308-Cell Division,
pubmed-meshheading:15807308-Coculture Techniques,
pubmed-meshheading:15807308-Collagen,
pubmed-meshheading:15807308-Culture Media, Conditioned,
pubmed-meshheading:15807308-Epidermal Growth Factor,
pubmed-meshheading:15807308-Fibroblasts,
pubmed-meshheading:15807308-Gels,
pubmed-meshheading:15807308-Hepatocyte Growth Factor,
pubmed-meshheading:15807308-Humans,
pubmed-meshheading:15807308-Ki-67 Antigen,
pubmed-meshheading:15807308-Mice,
pubmed-meshheading:15807308-Mouth Neoplasms,
pubmed-meshheading:15807308-Neoplasm Invasiveness,
pubmed-meshheading:15807308-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15807308-Protein Precursors,
pubmed-meshheading:15807308-Receptors, Interleukin-1,
pubmed-meshheading:15807308-Stromal Cells
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| pubmed:year |
2005
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| pubmed:articleTitle |
A new in vitro model for analyzing the biological behavior of well-differentiated squamous cell carcinoma.
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| pubmed:affiliation |
Department of Pathology and Biodefense, Division of Cellular and Molecular Pathology, Faculty of Medicine, Saga University, Saga 849-8501, Japan. satos3@med.saga-u.ac.jp
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| pubmed:publicationType |
Journal Article
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