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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-4-4
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pubmed:abstractText |
A choline-containing phospholipid (PL-4) in Methanopyrus kandleri cells was identified as archaetidylcholine, which has been described by Sprott et al. (1997). The PL-4 consisted of a variety of molecular species differing in hydrocarbon composition. Most of the PL-4 was acid-labile because of its allyl ether bond. The identity of PL-4 was confirmed by thin-layer chromatography followed by positive staining with Dragendorff reagent and fast-atom bombardment-mass spectrometry. A new method of LiAlH4 hydrogenolysis was developed to cleave allyl ether bonds and recover the corresponding hydrocarbons. We confirmed the validity of the LiAlH4 method in a study of the model compound synthetic unsaturated archaetidic acid (2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate). Saturated ether bonds were not cleaved by the LiAlH4 method. The hydrocarbons formed following LiAlH4 hydrogenolysis of PL-4 were identified by gas-liquid chromatography and mass spectrometry. Four kinds of hydrocarbons with one to four double bonds were detected: 47% of the hydrocarbons had four double bonds; 11% had three double bonds; 14% had two double bonds; 7% had one double bond; and 6% were saturated species. The molecular species composition of PL-4 was also estimated based on acid lability: 77% of the molecular species had two acid-labile hydrocarbons; 11% had one acid-labile and one acid-stable hydrocarbon; and 11% had two acid-stable hydrocarbons. To our knowledge, this is the first report of a specific chemical degradation method for the structural analysis of allyl ether phospholipid in archaea.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-10960477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-11540073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-13641241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-13671378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-13782493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-14173318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-17805900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-2380184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-3611039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-3734631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-5848277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15803650-9348086
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1472-3646
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
123-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15803650-Allyl Compounds,
pubmed-meshheading:15803650-Aluminum Compounds,
pubmed-meshheading:15803650-Choline,
pubmed-meshheading:15803650-Ethers,
pubmed-meshheading:15803650-Hot Temperature,
pubmed-meshheading:15803650-Kinetics,
pubmed-meshheading:15803650-Lithium Compounds,
pubmed-meshheading:15803650-Methanosarcinales,
pubmed-meshheading:15803650-Phospholipids,
pubmed-meshheading:15803650-Spectrometry, Mass, Fast Atom Bombardment
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pubmed:year |
2002
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pubmed:articleTitle |
Structural analysis by reductive cleavage with LiAlH4 of an allyl ether choline-phospholipid, archaetidylcholine, from the hyperthermophilic methanoarchaeon Methanopyrus kandleri.
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pubmed:affiliation |
Department of Chemistry, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
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pubmed:publicationType |
Journal Article
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