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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0023688,
umls-concept:C0031671,
umls-concept:C0086376,
umls-concept:C0086418,
umls-concept:C0205227,
umls-concept:C0597357,
umls-concept:C0597765,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1531127,
umls-concept:C1622340,
umls-concept:C2349975,
umls-concept:C2611812
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pubmed:issue |
6
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pubmed:dateCreated |
2005-5-26
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pubmed:abstractText |
Neuropeptides B and W (NPB and NPW) are regulatory peptides that act via two subtypes of G protein-coupled receptors, named GPR7 and GPR8. RT-PCR demonstrated the expression of these receptors in both zona glomerulosa and zona fasciculata-reticularis (ZF/R) cells of the human adrenal cortex. NPB and NPW did not affect aldosterone secretion from dispersed zona glomerulosa cells but enhanced cortisol production from ZF/R cells, NPB being more effective than NPW. NPB evoked sizable cAMP and inositol triphosphate responses from ZF/R cells, which were abrogated by the adenylate cyclase inhibitor SQ-22536 and the phospholipase C inhibitor U-73122, respectively. Cortisol response to NPB was lowered by either SQ-22536 and the protein kinase (PK) A inhibitor H-89 or U-73122 and the PKC inhibitor calphostin-C and abolished by the simultaneous exposure to H-89 and calphostin-C. NPW elicited only a rise in cAMP production from dispersed ZF/R cells, and its cortisol response was suppressed by both SQ-22536 and H-89. PreproNPB and preproNPW mRNAs were detected in human adrenal cortexes. We conclude that: 1) NPB and NPW exert a secretagogue action on human ZF/R cells, probably acting in an autocrine-paracrine manner; and 2) the effect of NPB is mediated by both the adenylate cyclase/PKA and the phospholipase C/PKC cascades, whereas that of NPW involves only the activation of the former signaling pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Aldosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrocortisone,
http://linkedlifedata.com/resource/pubmed/chemical/NPBWR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NPBWR2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NPW protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neuropeptide,
http://linkedlifedata.com/resource/pubmed/chemical/Steroid 11-beta-Hydroxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Steroid 17-alpha-Hydroxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/neuropeptide B
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-972X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3466-71
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pubmed:dateRevised |
2008-5-7
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pubmed:meshHeading |
pubmed-meshheading:15797961-Adenylate Cyclase,
pubmed-meshheading:15797961-Adrenal Cortex,
pubmed-meshheading:15797961-Aldosterone,
pubmed-meshheading:15797961-Cyclic AMP,
pubmed-meshheading:15797961-DNA Primers,
pubmed-meshheading:15797961-Humans,
pubmed-meshheading:15797961-Hydrocortisone,
pubmed-meshheading:15797961-Neuropeptides,
pubmed-meshheading:15797961-RNA, Messenger,
pubmed-meshheading:15797961-Receptors, G-Protein-Coupled,
pubmed-meshheading:15797961-Receptors, Neuropeptide,
pubmed-meshheading:15797961-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15797961-Signal Transduction,
pubmed-meshheading:15797961-Steroid 11-beta-Hydroxylase,
pubmed-meshheading:15797961-Steroid 17-alpha-Hydroxylase,
pubmed-meshheading:15797961-Type C Phospholipases,
pubmed-meshheading:15797961-Zona Fasciculata,
pubmed-meshheading:15797961-Zona Glomerulosa,
pubmed-meshheading:15797961-Zona Reticularis
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pubmed:year |
2005
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pubmed:articleTitle |
G protein receptors 7 and 8 are expressed in human adrenocortical cells, and their endogenous ligands neuropeptides B and w enhance cortisol secretion by activating adenylate cyclase- and phospholipase C-dependent signaling cascades.
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pubmed:affiliation |
Department of Human Anatomy and Physiology, Section of Anatomy, University of Padova, Via Gabelli 65, I-35121 Padova, Italy.
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pubmed:publicationType |
Journal Article
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