Linked Life Data

15796196

Source:http://linkedlifedata.com/resource/pubmed/id/15796196

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-3-30
pubmed:abstractText
Persistent, poorly healing wounds are a significant clinical problem in patients who have had previous irradiation. The pathology of chronic dermal ulcers is characterised by excessive proteolytic activity which degrades the extracellular matrix (required for cell migration) and growth factors and their receptors. Interestingly, the molecular basis of radiation-induced dermal wounds is poorly understood. The aim of this study was to investigate, by immunohistochemistry, the expression of the endothelial marker vWF, of angiogenic bFGF, VEGF and IL-8, of collagenases MMP-2 and MMP-9 and their inhibitors TIMP-1 and TIMP-2, in tissue samples from radiation-induced chronic dermal wounds and healthy control skin. Performing immunohistochemical detection of microvessels, an equivalent density of microvessels was observed within tissue samples from normal healthy skin and from radiation-induced non-healing cutaneous wounds. Investigation of angiogenic bFGF and VEGF demonstrated a decreased expression of both factors in the radiation-induced dermal wounds. The expression of angiogenic IL-8 was weak in both the healthy skin samples and the radiation-induced wounds. In addition, an increased expression of collagenases MMP-2 and MMP-9 protein within the radiation-induced wounds was demonstrated. While the expression of TIMP-1 showed no difference of expression between normal control skin and tissue samples from radiation-induced wounds, TIMP-2 expression was slightly increased compared to healthy controls. Our data suggest that radiation-induced dermal injuries often fail to heal because of decreased angiogenesis and persistently high concentrations of MMPs with an imbalance of their tissue inhibitors. The basic mechanisms of wound healing in radiation-induced dermal wounds at the molecular level need to be understood further for the development of innovative treatment strategies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0258-851X
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-50
pubmed:dateRevised
2007-5-2
pubmed:meshHeading
pubmed-meshheading:15796196-Biological Markers, pubmed-meshheading:15796196-Carcinoma, Squamous Cell, pubmed-meshheading:15796196-Chronic Disease, pubmed-meshheading:15796196-Fibroblast Growth Factor 2, pubmed-meshheading:15796196-Head and Neck Neoplasms, pubmed-meshheading:15796196-Humans, pubmed-meshheading:15796196-Immunohistochemistry, pubmed-meshheading:15796196-Interleukin-8, pubmed-meshheading:15796196-Matrix Metalloproteinases, pubmed-meshheading:15796196-Radiation Injuries, pubmed-meshheading:15796196-Skin, pubmed-meshheading:15796196-Tissue Inhibitor of Metalloproteinase-2, pubmed-meshheading:15796196-Tissue Inhibitor of Metalloproteinases, pubmed-meshheading:15796196-Vascular Endothelial Growth Factor A, pubmed-meshheading:15796196-Wound Healing, pubmed-meshheading:15796196-von Willebrand Factor
pubmed:articleTitle
Immunohistochemical analysis of radiation-induced non-healing dermal wounds of the head and neck.
pubmed:affiliation
Department of Otolaryngology, Head and Neck Surgery, University Hospital Mannheim, University of Heidelberg, Mannheim, Germany. frank.riedel@hno.ma.uni-heidelberg.de
pubmed:publicationType
Journal Article