Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2005-3-29
pubmed:abstractText
Viruses exploit a variety of cellular components to complete their life cycles, and it has become increasingly clear that use of host cell microtubules is a vital part of the infection process for many viruses. A variety of viral proteins have been identified that interact with microtubules, either directly or via a microtubule-associated motor protein. Here, we report that Ebola virus associates with microtubules via the matrix protein VP40. When transfected into mammalian cells, a fraction of VP40 colocalized with microtubule bundles and VP40 coimmunoprecipitated with tubulin. The degree of colocalization and microtubule bundling in cells was markedly intensified by truncation of the C terminus to a length of 317 amino acids. Further truncation to 308 or fewer amino acids abolished the association with microtubules. Both the full-length and the 317-amino-acid truncation mutant stabilized microtubules against depolymerization with nocodazole. Direct physical interaction between purified VP40 and tubulin proteins was demonstrated in vitro. A region of moderate homology to the tubulin binding motif of the microtubule-associated protein MAP2 was identified in VP40. Deleting this region resulted in loss of microtubule stabilization against drug-induced depolymerization. The presence of VP40-associated microtubules in cells continuously treated with nocodazole suggested that VP40 promotes tubulin polymerization. Using an in vitro polymerization assay, we demonstrated that VP40 directly enhances tubulin polymerization without any cellular mediators. These results suggest that microtubules may play an important role in the Ebola virus life cycle and potentially provide a novel target for therapeutic intervention against this highly pathogenic virus.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-10037788, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-10640541, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-10708448, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-10818356, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-10864502, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11044681, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11095724, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11118208, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11152484, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11163140, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11312656, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11333902, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11559815, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-1159898, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11601921, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11715020, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11715030, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11726971, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11799178, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11877482, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11878926, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11932414, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11964472, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-11967313, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-12134034, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-12525615, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-12681332, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-12787740, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-12857789, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-13679614, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-1396588, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-14592411, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-14645584, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-14673115, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-14963148, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-15117959, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-15254171, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-197697, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-219213, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-2212989, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-2324707, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-3016702, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-3142041, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-6296818, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-6302402, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-7629018, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-7699010, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-8009846, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-8107216, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-8241964, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-8523532, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-8645103, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-8834794, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-9658087, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-9658142, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-9841669, http://linkedlifedata.com/resource/pubmed/commentcorrection/15795257-9893375
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4709-19
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
More...