Source:http://linkedlifedata.com/resource/pubmed/id/15794943
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2005-3-29
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| pubmed:abstractText |
Phenylarsine oxide (PAO), a trivalent arsenical compound, stimulated [Ca2+]i elevation in rat neutrophils in a Ca2+-containing medium but caused no appreciable response in a Ca2+-free medium. PAO also induced external Mn2+ entry, which was inhibited by N-acetyl-L-cysteine (NAC), but failed to elicit any appreciable Ba2+ and Sr2+ entry. Pretreatment of neutrophils with thiol-reducing agents including dithiothreitol (DTT), NAC, 2,3-dimercapto-1-propanol (DMP), 2,3-dimercaptopropane-1-sulfonic acid (DMPS) and tris-(2-carboxyethyl)phosphine (TCEP), all greatly inhibited PAO-induced [Ca2+]i elevation. Addition of Ni2+ or La3+ followed by PAO stimulation also attenuated the Ca2+ signals in a concentration-dependent manner. PAO had no significant effect on the production of reactive oxygen intermediates (ROI) and nitric oxide (NO) nor did it decrease cellular low molecular weight thiols levels. PAO-induced [Ca2+]i elevation was significantly inhibited by 1-[6-[17beta-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione (U-73122), the inhibitor of phospholipase C-coupled processes, genistein, a general tyrosine kinase inhibitor, phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, calyculin A, a cortical actin stabilizer, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002), a phosphoinositide 3-kinase inhibitor, 1-[beta-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1H-imidazole (SKF-96365), and cis-N-(2-phenylcyclopentyl)azacyclotridec-1-en-2-amine (MDL-12,330A), the blockers of receptor-gated and store-operated Ca2+ channels, whereas there was no appreciable effect exerted by aristolochic acid, a phospholipase A2 inhibitor, 7-nitroindazole and N-(3-aminomethyl)benzylacetamidine (1400W), the blockers of NO synthase, and by suspension in a Na+-deprived medium. In contrast, 2-aminoethoxydiphenyl borane (2-APB), the blocker of IP3 receptor and Ca2+ influx, enhanced the PAO-induced response. PAO had no effect on the plasma membrane Ca2+-ATPase (PMCA) activity in the pharmacological isolated neutrophil preparation and the neutrophil membrane fractions. These results indicate that PAO stimulates [Ca2+]i rise in rat neutrophils mainly through the oxidation of vicinal thiol groups on the cell surface membrane to activation of a non-store operated Ca2+ entry (non-SOCE) without affecting the activity of PMCA and the plasmalemmal Na+/Ca2+ exchanger.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/7-nitroindazole,
http://linkedlifedata.com/resource/pubmed/chemical/Aristolochic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Arsenicals,
http://linkedlifedata.com/resource/pubmed/chemical/Barium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Carbamates,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Indazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Manganese,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Strontium,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/amprenavir,
http://linkedlifedata.com/resource/pubmed/chemical/aristolochic acid I,
http://linkedlifedata.com/resource/pubmed/chemical/cyclopiazonic acid,
http://linkedlifedata.com/resource/pubmed/chemical/oxophenylarsine
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0006-2952
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
69
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1225-34
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:15794943-Animals,
pubmed-meshheading:15794943-Aorta, Abdominal,
pubmed-meshheading:15794943-Aristolochic Acids,
pubmed-meshheading:15794943-Arsenicals,
pubmed-meshheading:15794943-Barium,
pubmed-meshheading:15794943-Calcium,
pubmed-meshheading:15794943-Calcium Signaling,
pubmed-meshheading:15794943-Calcium-Transporting ATPases,
pubmed-meshheading:15794943-Carbamates,
pubmed-meshheading:15794943-Cell Membrane,
pubmed-meshheading:15794943-Enzyme Inhibitors,
pubmed-meshheading:15794943-Indazoles,
pubmed-meshheading:15794943-Indoles,
pubmed-meshheading:15794943-Male,
pubmed-meshheading:15794943-Manganese,
pubmed-meshheading:15794943-NADPH Oxidase,
pubmed-meshheading:15794943-Neutrophils,
pubmed-meshheading:15794943-Nitric Oxide,
pubmed-meshheading:15794943-Rats,
pubmed-meshheading:15794943-Rats, Sprague-Dawley,
pubmed-meshheading:15794943-Reactive Oxygen Species,
pubmed-meshheading:15794943-Strontium,
pubmed-meshheading:15794943-Sulfonamides
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| pubmed:year |
2005
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| pubmed:articleTitle |
Stimulation of intracellular Ca2+ elevation in neutrophils by thiol-oxidizing phenylarsine oxide.
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| pubmed:affiliation |
Department of Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan, ROC. w1994@vghtc.gov.tw
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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