Source:http://linkedlifedata.com/resource/pubmed/id/15794089
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2005-3-29
|
| pubmed:abstractText |
Inter-laboratory evaluation studies were conducted for the ELISA methods for allergic substances (buckwheat). Extracts of snack, bun and udon spiked with buckwheat standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate at 10 laboratories. Coefficients of variation (CVs) of the ELISA methods using a Buckwheat Protein ELISA Kit (Buckwheat kit) and a FASTKIT Buckwheat ELISA kit (Buckwheat ELISA kit) were mostly below 10%. Mean recoveries of the buckwheat standard protein from the food extracts were over 40% in the two ELISA methods. Repeatability relative standard deviations of buckwheat standard protein in three food extracts were in the ranges of 6.8-78.5% and 5.0-33.9% for the Buckwheat kit and the Buckwheat ELISA kit, respectively. Reproducibility relative standard deviations of buckwheat standard protein in three food extracts were 11.9-69.5% and 16.5-34.1% for the Buckwheat kit and the Buckwheat ELISA kit, respectively. The detection limits of both ELISA methods were 1 ng/mL in sample solutions. These results suggest that the notified ELISA methods are reliable and reproducible for the inspection of buckwheat protein levels in extracts of snack, bun and udon.
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| pubmed:language |
jpn
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0015-6426
|
| pubmed:author |
pubmed-author:AkiyamaHiroshiH,
pubmed-author:ArakawaFumihiroF,
pubmed-author:HarikaiNaokiN,
pubmed-author:HonjohTsutomuT,
pubmed-author:IijimaKenK,
pubmed-author:ImamuraTomoakiT,
pubmed-author:KatoHisashiH,
pubmed-author:MaitaniTamioT,
pubmed-author:MamegoshiShinichiS,
pubmed-author:MatsudaRiekoR,
pubmed-author:MizuguchiYamatoY,
pubmed-author:MorimatsuFumikiF,
pubmed-author:MuraokaShirooS,
pubmed-author:NakamuraKenjinK,
pubmed-author:NishiharaRikukaR,
pubmed-author:OgasawaraTakeshiT,
pubmed-author:SakataKozueK,
pubmed-author:SatoHidetakaH,
pubmed-author:TakahataYoshihisaY,
pubmed-author:ToyodaMasatakeM,
pubmed-author:WataiMasatoshiM,
pubmed-author:WatanabeHirokoH,
pubmed-author:WatanabeTakahiroT,
pubmed-author:YamakawaHirohitoH,
pubmed-author:YamamotoMihoM,
pubmed-author:YamauchiAtsushiA,
pubmed-author:YoshikawaReijiR
|
| pubmed:issnType |
Print
|
| pubmed:volume |
45
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
313-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15794089-Allergens,
pubmed-meshheading:15794089-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15794089-Fagopyrum,
pubmed-meshheading:15794089-Food Analysis,
pubmed-meshheading:15794089-Japan,
pubmed-meshheading:15794089-Laboratories,
pubmed-meshheading:15794089-Reproducibility of Results,
pubmed-meshheading:15794089-Sensitivity and Specificity
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
[Inter-laboratory evaluation studies for establishment of notified ELISA methods for allergic substances (buckwheat)].
|
| pubmed:affiliation |
National Institute of Health Sciences, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
English Abstract,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|