rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2005-12-2
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pubmed:databankReference |
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pubmed:abstractText |
Rotavirus genotyping is performed by using reverse transcription PCR with type-specific-primers. Because the high rotavirus mutation rate generates an extensive genomic variation, different G-type-specific primer sets are applied in different geographical locations. In Bangladesh, a significant proportion (36.9%) of the rotavirus strains isolated in 2002 could not be G-typed using the routinely used primer set. To investigate the reason why the strains were untypeable, nucleotide sequencing of the VP7 genes was performed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15790408-10325342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15790408-10568765,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15790408-9672646
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1743-422X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2005
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pubmed:articleTitle |
Typing of human rotaviruses: nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure.
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pubmed:affiliation |
ICDDR,B: Centre for Health and Population Research, Mohakhali, Dhaka-1212, Bangladesh. mustafizur.rahman@uz.kuleuven.ac.be
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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