Source:http://linkedlifedata.com/resource/pubmed/id/15790072
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2005-3-25
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| pubmed:abstractText |
In this preliminary report we show that a 29 kDa surface protein of Listeria monocytogenes EGD removed from cells with 4 M LiCl has peptidoglycan (murein) hydrolyzing activity, as revealed by zymographic analysis using Bacillus subtilis murein and heat-killed Micrococcus luteus cells casted in the gel. Following two-dimensional electrophoresis, the protein was electroblotted to PVDF membrane and its identity (FlaA) was revealed by sequencing. Peptidoglycan hydrolysing activity of FlaA purified by FPLC on Mono-S Sepharose against labelled Escherichia coli murein was demonstrated.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1733-1331
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
53
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
237-41
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| pubmed:dateRevised |
2005-11-23
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| pubmed:meshHeading |
pubmed-meshheading:15790072-Chromatography, Affinity,
pubmed-meshheading:15790072-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:15790072-Escherichia coli,
pubmed-meshheading:15790072-Flagellin,
pubmed-meshheading:15790072-Listeria monocytogenes,
pubmed-meshheading:15790072-N-Acetylmuramoyl-L-alanine Amidase,
pubmed-meshheading:15790072-Peptidoglycan
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| pubmed:year |
2004
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| pubmed:articleTitle |
Murein-hydrolyzing activity of flagellin FlaA of Listeria monocytogenes.
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| pubmed:affiliation |
Department of Bacterial Physiology, Institute of Microbiology, Warsaw University Miecznikowa 1, 02-096 Warsaw, Poland. magdapop@biol.uw.edu.pl
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| pubmed:publicationType |
Journal Article
|