Linked Life Data

1578900

Source:http://linkedlifedata.com/resource/pubmed/id/1578900

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-6-9
pubmed:abstractText
The biotin derivative, N-(2-aminoethyl) biotinamide hydrochloride, or Neurobiotin, has been shown recently to be a useful marker for intracellular and anterograde tracing. The properties of Neurobiotin as a tracer were further examined in this study by making pressure injections into different regions of the cerebral cortex or the rostral neostriatum of rats or by incubating striatal cells in culture with the tracer. Results showed extensive anterograde transport of Neurobiotin in cortical axons and terminals within the neostriatum 2-70 h after single or multiple cortical injections of the tracer. Similarly, profuse axonal projections to the medial portion of the globus pallidus were seen after an injection of Neurobiotin into the rostral neostriatum. Transneuronal labeling of medium-size neostriatal neurons was observed following injections of Neurobiotin into the prefrontal cortex. At the ultrastructural level, anterogradely labeled cortical axon terminals and transneuronally labeled neurons were readily identified in the caudate-putamen by the presence of both fine particulate and large punctate reaction products. Retrograde fillings of neurons resembling a Golgi-impregnation were seen in the ventral posterior complex of the thalamus after injections in the sensorimotor cortex. Neurons in the medial globus pallidus were also retrogradely labeled following tracer injections in the rostral caudate-putamen. Finally, Neurobiotin was readily and selectively taken up by striatal neurons in culture, where it extensively labeled somata and neurites. These results show that Neurobiotin is a versatile new tracer, which can be potentially useful for the study of neuronal organization in vivo and in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Neurobiotin, a useful neuroanatomical tracer for in vivo anterograde, retrograde and transneuronal tract-tracing and for in vitro labeling of neurons.
pubmed:affiliation
Department of Neurology, Massachusetts General Hospital, Boston.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.