Linked Life Data

15788374

Source:http://linkedlifedata.com/resource/pubmed/id/15788374

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-3-24
pubmed:abstractText
Various particulate matter (PM) samples were tested for their adjuvant potency in an animal model of allergy (ovalbumin) in the European Union study entitled Respiratory Allergy and Inflammation Due to Ambient Particles. Coarse and fine ambient particles were collected during spring, summer, and winter in Rome, Oslo, Lodz, Amsterdam, and De Zilk. De Zilk, at the Dutch seaside, has mainly westerly winds and served as a negative pollution control. EHC-93 (Ottawa dust) was used as a positive control. We studied the adjuvant potency of the particle antibody responses to ovalbumin and histopathological changes in the lung. After a sensitization phase by coexposure to EHC-93 and ovalbumin, the antibody response to ovalbumin and inflammatory responses in the lung were huge. There was more adjuvant activity in reaction to 9-mg/ml samples than to 3-mg/ml samples. A best-fit analysis of these samples shows that the ambient coarse and fine particles at these sites, in combination with allergens, have severe to mild adjuvant activity in the order Lodz, Rome, Oslo, and Amsterdam. A high dose of the fine fraction was more potent than a high dose of the coarse fraction, except at De Zilk, where the reverse was true. Spring and winter PM was more potent than summer PM. Depending on the site, either a water-soluble or a water-insoluble fraction was responsible for the adjuvant activity. A concentration of 3 mg/ml is effective for screening high-activity samples, as is a concentration of 9 mg/ml for screening low-activity samples in the ovalbumin-mouse model.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0895-8378
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15788374-Adjuvants, Immunologic, pubmed-meshheading:15788374-Air Pollutants, pubmed-meshheading:15788374-Animals, pubmed-meshheading:15788374-Bronchoalveolar Lavage Fluid, pubmed-meshheading:15788374-Cytokines, pubmed-meshheading:15788374-Disease Models, Animal, pubmed-meshheading:15788374-Dust, pubmed-meshheading:15788374-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:15788374-Europe, pubmed-meshheading:15788374-Immunoglobulin E, pubmed-meshheading:15788374-Immunoglobulin G, pubmed-meshheading:15788374-L-Lactate Dehydrogenase, pubmed-meshheading:15788374-Lung, pubmed-meshheading:15788374-Macrophages, Alveolar, pubmed-meshheading:15788374-Male, pubmed-meshheading:15788374-Mice, pubmed-meshheading:15788374-Mice, Inbred BALB C, pubmed-meshheading:15788374-Ovalbumin, pubmed-meshheading:15788374-Particle Size, pubmed-meshheading:15788374-Respiratory Hypersensitivity, pubmed-meshheading:15788374-Seasons, pubmed-meshheading:15788374-Solubility
pubmed:year
2005
pubmed:articleTitle
Dose dependency of adjuvant activity of particulate matter from five European sites in three seasons in an ovalbumin-mouse model.
pubmed:affiliation
Laboratory for Toxicology, Pathology, and Genetics, National Institute of Public Health and the Environment, Bilthoven, the Netherlands. p.steerenberg@rivm.nl
pubmed:publicationType
Journal Article, Comparative Study