Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-3-22
pubmed:abstractText
Previous work has demonstrated that iron-dependent variations in the steady-state concentration and translatability of sodB mRNA are modulated by the small regulatory RNA RyhB, the RNA chaperone Hfq and RNase E. In agreement with the proposed role of RNase E, we found that the decay of sodB mRNA is retarded upon inactivation of RNase E in vivo, and that the enzyme cleaves within the sodB 5'-untranslated region (5'-UTR) in vitro, thereby removing the 5' stem-loop structure that facilitates Hfq and ribosome binding. Moreover, RNase E cleavage can also occur at a cryptic site that becomes available upon sodB 5'-UTR/RyhB base pairing. We show that while playing an important role in facilitating the interaction of RyhB with sodB mRNA, Hfq is not tightly retained by the RyhB-sodB mRNA complex and can be released from it through interaction with other RNAs added in trans. Unlike turnover of sodB mRNA, RyhB decay in vivo is mainly dependent on RNase III, and its cleavage by RNase III in vitro is facilitated upon base pairing with the sodB 5'-UTR. These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-10090722, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-10200269, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-10809669, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-10823925, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-10926508, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-11179343, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-11222598, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-11251852, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-11282473, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-11804582, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-11882724, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-11917098, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-12051911, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-12119387, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-12230973, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-12495429, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-12975324, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-14522943, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-14561880, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-14617150, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-14623313, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-14739933, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-15620655, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-1712252, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-1725264, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-1730408, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-2002510, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-2423846, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-2467619, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-2481042, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-2695390, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-3280138, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-3308454, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-6330047, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-6387508, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-6864790, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-7526223, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-7530223, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-7542588, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-790651, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-8001821, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-8364098, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-8610017, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-8632981, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-8662734, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-8995304, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-9632264, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-9735284, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-9790196, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-9826663, http://linkedlifedata.com/resource/pubmed/commentcorrection/15781494-9932452
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/5' Untranslated Regions, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases, http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hfq protein, E coli, http://linkedlifedata.com/resource/pubmed/chemical/Host Factor 1 Protein, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Untranslated, http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease III, http://linkedlifedata.com/resource/pubmed/chemical/SodB protein, Bacteria, http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase, http://linkedlifedata.com/resource/pubmed/chemical/ribonuclease E
pubmed:status
MEDLINE
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1678-89
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
More...