Linked Life Data

15781297

Source:http://linkedlifedata.com/resource/pubmed/id/15781297

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-3-22
pubmed:abstractText
Chronic exposure to cadmium (Cd) results in bicarbonaturia, leading to metabolic acidosis. To elucidate the mechanism(s) by which renal bicarbonate reabsorption is inhibited, we investigated changes in renal transporters and enzymes associated with bicarbonate reabsorption in Cd-intoxicated rats. Cd intoxication was induced by subcutaneous injections of CdCl(2) (2 mg Cd/kg per day) for 3 weeks. Cd intoxication resulted in a significant reduction in V(max) of Na(+)/H(+) antiport with no changes in K(Na) in the renal cortical brush-border membrane vesicles (BBMV). Western blotting of BBM proteins and indirect immunohistochemistry in renal tissue sections, using an antibody against Na(+)/H(+) exchange-3 (NHE3), showed a diminished expression of NHE3 protein in the BBM. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that NHE3 mRNA expression was reduced in the renal cortex. The activity of carbonic anhydrase IV (CA IV) in BBM was not changed. The protein abundance of Na(+)-HCO(3)(-) cotransporter-1 (NBC1) in whole kidney membrane fractions was slightly attenuated, whereas that of the Na(+)-K(+)-ATPase alpha-subunit was markedly elevated in Cd-intoxicated animals. These results indicate that Cd intoxication impairs NHE3 expression in the proximal tubule, thereby reducing the capacity for bicarbonate reabsorption, leading to bicarbonaturia in an intact animal.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
204
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Inhibition of renal Na+/H+ exchange in cadmium-intoxicated rats.
pubmed:affiliation
Department of Physiology, Kosin University College of Medicine, Busan 602-702, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't