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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-3-22
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pubmed:abstractText |
SWI/SNF- and ISWI-based complexes have distinct yet overlapping chromatin-remodeling activities in vitro and perform different roles in vivo. This leads to the hypothesis that the distinct remodeling functions of these complexes are specifically required for distinct biological tasks. By creating and characterizing chimeric proteins of BRG1 and SNF2h, the motor proteins of human SWI/SNF- and ISWI-based complexes, respectively, we found that a region that includes the ATPase domain specifies the outcome of the remodeling reaction in vitro. A chimeric protein based on BRG1 but containing the SNF2h ATPase domain formed an intact SWI/SNF complex that remodeled like SNF2h. This altered-function complex was active for remodeling and could stimulate expression from some, but not all, SWI/SNF responsive promoters in vivo. Thus, we were able to separate domains of BRG1 responsible for function from those responsible for SWI/SNF complex formation and demonstrate that remodeling functions are not interchangeable in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleosomes,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucocorticoid,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SMARCA4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/SMARCA5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
805-15
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15780937-Adenosine Triphosphatases,
pubmed-meshheading:15780937-Adrenal Gland Neoplasms,
pubmed-meshheading:15780937-Chromatin,
pubmed-meshheading:15780937-Chromatin Assembly and Disassembly,
pubmed-meshheading:15780937-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:15780937-DNA Helicases,
pubmed-meshheading:15780937-Humans,
pubmed-meshheading:15780937-Nuclear Proteins,
pubmed-meshheading:15780937-Nucleosomes,
pubmed-meshheading:15780937-Promoter Regions, Genetic,
pubmed-meshheading:15780937-Protein Structure, Tertiary,
pubmed-meshheading:15780937-Receptors, Glucocorticoid,
pubmed-meshheading:15780937-Recombinant Fusion Proteins,
pubmed-meshheading:15780937-Transcription, Genetic,
pubmed-meshheading:15780937-Transcription Factors,
pubmed-meshheading:15780937-Transcriptional Activation,
pubmed-meshheading:15780937-Tumor Cells, Cultured,
pubmed-meshheading:15780937-Zinc Fingers
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pubmed:year |
2005
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pubmed:articleTitle |
Swapping function of two chromatin remodeling complexes.
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pubmed:affiliation |
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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