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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-5-30
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pubmed:abstractText |
The present study underlines the importance of phospholipase A2 (PLA2)- and lipoxygenase (LO)-mediated signaling processes in the regulation of inducible nitric oxide synthase (iNOS) gene expression. In glial cells, lipopolysaccharide (LPS) induced the activities of PLA2 (calcium-independent PLA2; iPLA2 and cytosolic PLA2; cPLA2) as well as gene expression of iNOS. The inhibition of cPLA2 by methyl arachidonyl fluorophosphates (MAFP) or antisense oligomer against cPLA2 and inhibition of iPLA2 by bromoenol lactone reduced the LPS-induced iNOS gene expression and NFkappaB activation. In addition, the inhibition of LO by nordihydroguaiaretic acid (NDGA; general LO inhibitor) or MK886 (5-LO inhibitor), but not baicalein (12-LO inhibitor), completely abrogated the LPS-induced iNOS expression. Because NDGA could abrogate the LPS-induced activation of NFkappaB, while MK886 had no effect on it, LO-mediated inhibition of iNOS gene induction by LPS may involve an NFkappaB-dependent or -independent (by 5-LO) pathway. In contrast to LO, however, the cyclooxygenase (COX) may not be involved in the regulation of LPS-mediated induction of iNOS gene because COX inhibition by indomethacin (general COX inhibitor), SC560 (COX-1 inhibitor), and NS398 (COX-2 inhibitor) affected neither the LPS-induced iNOS expression nor activation of NFkappaB. These results indicate a role for cPLA2 and iPLA2 in LPS-mediated iNOS gene induction in glial cells and the involvement of LO in these reactions.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 5-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0894-1491
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pubmed:author |
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pubmed:copyrightInfo |
Copyright (c) 2005 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13-21
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15779087-Animals,
pubmed-meshheading:15779087-Arachidonate 5-Lipoxygenase,
pubmed-meshheading:15779087-Arachidonic Acid,
pubmed-meshheading:15779087-Blotting, Northern,
pubmed-meshheading:15779087-Cell Line,
pubmed-meshheading:15779087-Cell Line, Tumor,
pubmed-meshheading:15779087-Cell Nucleus,
pubmed-meshheading:15779087-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:15779087-Enzyme Induction,
pubmed-meshheading:15779087-Enzyme Inhibitors,
pubmed-meshheading:15779087-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:15779087-Genes, Reporter,
pubmed-meshheading:15779087-Isoenzymes,
pubmed-meshheading:15779087-Lipopolysaccharides,
pubmed-meshheading:15779087-Lipoxygenase,
pubmed-meshheading:15779087-NF-kappa B,
pubmed-meshheading:15779087-Neuroglia,
pubmed-meshheading:15779087-Nitric Oxide Synthase,
pubmed-meshheading:15779087-Nitric Oxide Synthase Type II,
pubmed-meshheading:15779087-Phospholipases A,
pubmed-meshheading:15779087-Phospholipases A2,
pubmed-meshheading:15779087-RNA,
pubmed-meshheading:15779087-Rats,
pubmed-meshheading:15779087-Transcriptional Activation,
pubmed-meshheading:15779087-Transfection
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pubmed:year |
2005
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pubmed:articleTitle |
Involvement of phospholipase A2 and lipoxygenase in lipopolysaccharide-induced inducible nitric oxide synthase expression in glial cells.
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pubmed:affiliation |
Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina 29425, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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