Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
14
|
| pubmed:dateCreated |
1992-6-9
|
| pubmed:abstractText |
A strong positive element within the proximal promoter region of the human beta-myosin heavy chain (beta-MHC) gene that is required for high level expression in primary cultures of fetal rat heart cells was localized by transient assays and DNase I footprinting to positions- 277/-298. Using gel shift studies, this sequence was found to bind specifically at high affinity (Kd approximately 4 x 10(-9) M) to a transcriptional factor (beta F1) found in nuclear extracts from rabbit heart. Dimethyl sulfate interference studies suggested that beta F1 may bind as a dimer to two hexameric imperfect direct repeats containing the consensus sequence 5'-(C/G)-T-G-(T/A)-G-G-3'. Gel shift analyses suggested that beta F1 is related to the M-CAT factor, which is known to control muscle-specific expression of the cardiac troponin T gene. A clustered mutation of the region between the putative binding half-sites and within the "M-CAT"-like domain abolished beta-MHC promoter activity. The sequence of the positive element also contains binding motifs for several transcriptional factors that regulate viral and cellular genes, including AP4, AP5, TEF-1, and MyoD-like proteins. When multiple copies of the beta-MHC element were inserted downstream from the transcriptional initiation site of the thymidine kinase gene, it did not act as a classical enhancer, showing some dependence upon orientation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
267
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
9917-24
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:1577822-Animals,
pubmed-meshheading:1577822-Base Sequence,
pubmed-meshheading:1577822-Cell Line,
pubmed-meshheading:1577822-Cell Nucleus,
pubmed-meshheading:1577822-Cells, Cultured,
pubmed-meshheading:1577822-Cloning, Molecular,
pubmed-meshheading:1577822-Deoxyribonuclease I,
pubmed-meshheading:1577822-Enhancer Elements, Genetic,
pubmed-meshheading:1577822-Fetus,
pubmed-meshheading:1577822-HeLa Cells,
pubmed-meshheading:1577822-Heart,
pubmed-meshheading:1577822-Humans,
pubmed-meshheading:1577822-Kinetics,
pubmed-meshheading:1577822-Liver,
pubmed-meshheading:1577822-Molecular Sequence Data,
pubmed-meshheading:1577822-Mutagenesis, Site-Directed,
pubmed-meshheading:1577822-Myosins,
pubmed-meshheading:1577822-Nuclear Proteins,
pubmed-meshheading:1577822-Oligodeoxyribonucleotides,
pubmed-meshheading:1577822-Plasmids,
pubmed-meshheading:1577822-Polymerase Chain Reaction,
pubmed-meshheading:1577822-Promoter Regions, Genetic,
pubmed-meshheading:1577822-Rabbits,
pubmed-meshheading:1577822-Rats,
pubmed-meshheading:1577822-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1577822-Restriction Mapping,
pubmed-meshheading:1577822-Transcription, Genetic
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Characterization of a strong positive cis-acting element of the human beta-myosin heavy chain gene in fetal rat heart cells.
|
| pubmed:affiliation |
University Heart Center, Tucson, Arizona.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|