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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-5-30
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pubmed:abstractText |
Notch signaling plays a pivotal role in numerous cell fate determination events during development, and therefore its regulation has been studied intensively. MSX2-interacting nuclear target protein (MINT) modifies the Notch signaling by interacting with and inhibiting the downstream transcription factor RBP-J/CBF-1 of Notch. In this study, by a yeast two hybrid screening, we found that the C terminal fragment of MINT interacted with each other. We confirmed the interaction between two MINT C terminal fragments both in vitro and in vivo. We further demonstrated that the overexpression of the C terminal fragment of MINT cancelled its inhibitory effect on the transactivation of an RBP-J-dependent promoter by Notch. These results suggest that MINT may form a dimer or multimer in cells through its C terminus, and that the C terminal fragment of MINT may work as its dominant-negative version.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/APBA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin J Recombination...,
http://linkedlifedata.com/resource/pubmed/chemical/MSX2 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RBPJ protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Notch,
http://linkedlifedata.com/resource/pubmed/chemical/Spen protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
1729
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
50-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15777657-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:15777657-Animals,
pubmed-meshheading:15777657-COS Cells,
pubmed-meshheading:15777657-Cercopithecus aethiops,
pubmed-meshheading:15777657-DNA-Binding Proteins,
pubmed-meshheading:15777657-Gene Expression Regulation,
pubmed-meshheading:15777657-Homeodomain Proteins,
pubmed-meshheading:15777657-Humans,
pubmed-meshheading:15777657-Immunoglobulin J Recombination Signal Sequence-Binding...,
pubmed-meshheading:15777657-Membrane Proteins,
pubmed-meshheading:15777657-Nerve Tissue Proteins,
pubmed-meshheading:15777657-Nuclear Proteins,
pubmed-meshheading:15777657-Promoter Regions, Genetic,
pubmed-meshheading:15777657-Protein Structure, Tertiary,
pubmed-meshheading:15777657-Receptors, Notch,
pubmed-meshheading:15777657-Signal Transduction,
pubmed-meshheading:15777657-Transcription, Genetic
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pubmed:year |
2005
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pubmed:articleTitle |
The C terminus of MINT forms homodimers and abrogates MINT-mediated transcriptional repression.
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pubmed:affiliation |
Department of Medical Genetics and Developmental Biology, State Key Laboratory of GI Cancer Biology, Fourth Military Medical University, Xian 710032, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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