Linked Life Data

15775970

Source:http://linkedlifedata.com/resource/pubmed/id/15775970

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-5-19
pubmed:abstractText
Apoptosis-inducing factor (AIF) is a mitochondrial intermembrane flavoprotein that is translocated to the nucleus in response to proapoptotic stimuli, where it induces nuclear apoptosis. Here we show that AIF is synthesized as an approximately 67-kDa preprotein with an N-terminal extension and imported into mitochondria, where it is processed to the approximately 62-kDa mature form. Topology analysis revealed that mature AIF is a type-I inner membrane protein with the N-terminus exposed to the matrix and the C-terminal portion to the intermembrane space. Upon induction of apoptosis, processing of mature AIF to an approximately 57-kDa form occurred caspase-independently in the intermembrane space, releasing the processed form into the cytoplasm. Bcl-2 or Bcl-XL inhibited both these events. These findings indicate that AIF release from mitochondria occurs by a two-step process: detachment from the inner membrane by apoptosis-induced processing in the intermembrane space and translocation into the cytoplasm. The results also suggest the presence of a unique protease that is regulated by proapoptotic stimuli in caspase-independent cell death.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-10087920, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-10822379, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-10929711, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-10929712, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11259394, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11279485, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11314271, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11360178, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11470436, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11583623, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11604410, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11606597, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-11818574, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12147675, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12198487, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12353028, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12408857, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12486111, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12600312, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12668678, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12774122, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12901865, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-12941691, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-1328170, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-1328858, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-14990999, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-15096522, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-15590628, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-1991446, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-3521588, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-8266095, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-8389693, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-8689682, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-8861942, http://linkedlifedata.com/resource/pubmed/commentcorrection/15775970-9989411
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1375-86
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:15775970-Apoptosis, pubmed-meshheading:15775970-Apoptosis Inducing Factor, pubmed-meshheading:15775970-Cell Fractionation, pubmed-meshheading:15775970-DNA, Complementary, pubmed-meshheading:15775970-Flavoproteins, pubmed-meshheading:15775970-Green Fluorescent Proteins, pubmed-meshheading:15775970-HeLa Cells, pubmed-meshheading:15775970-Humans, pubmed-meshheading:15775970-Immunohistochemistry, pubmed-meshheading:15775970-Membrane Proteins, pubmed-meshheading:15775970-Microscopy, Fluorescence, pubmed-meshheading:15775970-Mitochondria, pubmed-meshheading:15775970-Models, Biological, pubmed-meshheading:15775970-Protein Processing, Post-Translational, pubmed-meshheading:15775970-Protein Transport, pubmed-meshheading:15775970-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:15775970-Transfection, pubmed-meshheading:15775970-bcl-X Protein
pubmed:year
2005
pubmed:articleTitle
Export of mitochondrial AIF in response to proapoptotic stimuli depends on processing at the intermembrane space.
pubmed:affiliation
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't