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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2005-3-15
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pubmed:abstractText |
Physical, genetic, and chemical-genetic interactions centered on the conserved chaperone Hsp90 were mapped at high resolution in yeast using systematic proteomic and genomic methods. Physical interactions were identified using genome-wide two hybrid screens combined with large-scale affinity purification of Hsp90-containing protein complexes. Genetic interactions were uncovered using synthetic genetic array technology and by a microarray-based chemical-genetic screen of a set of about 4700 viable yeast gene deletion mutants for hypersensitivity to the Hsp90 inhibitor geldanamycin. An extended network, consisting of 198 putative physical interactions and 451 putative genetic and chemical-genetic interactions, was found to connect Hsp90 to cofactors and substrates involved in a wide range of cellular functions. Two novel Hsp90 cofactors, Tah1 (YCR060W) and Pih1 (YHR034C), were also identified. These cofactors interact physically and functionally with the conserved AAA(+)-type DNA helicases Rvb1/Rvb2, which are key components of several chromatin remodeling factors, thereby linking Hsp90 to epigenetic gene regulation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Coenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/HSP82 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/HSP90 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Molecular Chaperones,
http://linkedlifedata.com/resource/pubmed/chemical/Quinones,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/RVB1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/RVB2 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/geldanamycin
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0092-8674
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pubmed:author |
pubmed-author:BooneCharlesC,
pubmed-author:CagneyGerardG,
pubmed-author:DaveyMikeM,
pubmed-author:EmiliAndrewA,
pubmed-author:FOXP BPB,
pubmed-author:GreenblattJackJ,
pubmed-author:HouryWalid AWA,
pubmed-author:HsuYa-ChiehYC,
pubmed-author:KaplanekPiaP,
pubmed-author:KroganNevanN,
pubmed-author:ParsonsAinslie BAB,
pubmed-author:TongAmyA,
pubmed-author:ZhaoRongminR
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
120
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
715-27
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15766533-Adenosine Triphosphatases,
pubmed-meshheading:15766533-Benzoquinones,
pubmed-meshheading:15766533-Chromosome Mapping,
pubmed-meshheading:15766533-Coenzymes,
pubmed-meshheading:15766533-DNA Helicases,
pubmed-meshheading:15766533-Drug Resistance, Fungal,
pubmed-meshheading:15766533-Epigenesis, Genetic,
pubmed-meshheading:15766533-Gene Expression Regulation, Fungal,
pubmed-meshheading:15766533-Genome, Fungal,
pubmed-meshheading:15766533-HSP90 Heat-Shock Proteins,
pubmed-meshheading:15766533-Lactams, Macrocyclic,
pubmed-meshheading:15766533-Mass Spectrometry,
pubmed-meshheading:15766533-Molecular Chaperones,
pubmed-meshheading:15766533-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15766533-Proteomics,
pubmed-meshheading:15766533-Quinones,
pubmed-meshheading:15766533-RNA Helicases,
pubmed-meshheading:15766533-Saccharomyces cerevisiae,
pubmed-meshheading:15766533-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:15766533-Transcription Factors,
pubmed-meshheading:15766533-Two-Hybrid System Techniques
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pubmed:year |
2005
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pubmed:articleTitle |
Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone.
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pubmed:affiliation |
Department of Biochemistry, Medical Sciences Building, 1 King's College Circle, University of Toronto, Toronto, ON, M5S 1A8, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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