Linked Life Data

15765542

Source:http://linkedlifedata.com/resource/pubmed/id/15765542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-4-4
pubmed:abstractText
Pseudomonas aeruginosa (PA) is associated with a distinctive smell produced by a combination of volatile compounds (VCs). Selected ion flow tube mass spectrometry (SIFT-MS) provides a novel and rapid methodology for rapid, accurate detection of trace quantities (parts per billion; ppb) of VCs in air. We studied the VCs produced by different isolates of PA cultures in vitro from patients with cystic fibrosis. Twenty-one patients with cystic fibrosis provided sputum and cough swab samples for culture. These were used to inoculate blood agar (BA) and Pseudomonas-selective media (PSM). These plates were incubated for 48 hr at 37 degrees C inside sealed plastic bags. The air surrounding the samples after 48 hr (headspace) was analyzed using SIFT-MS. PA growth was commonly associated with the production of significant quantities of VCs, notably hydrogen cyanide gas (HCN). This was detectable in the headspace of 15/22 of PA-positive samples. In contrast, it was only seen in the headspace of 1/13 control samples (6 sterile plates and 7 plates with only mixed upper respiratory tract flora). The concentration of HCN was significantly higher above PA-positive samples than above other bacterial growth (P < 0.01), and in our study, levels of HCN greater than 100 ppb were a sensitive (68%) and highly specific (100%) biomarker of PA. SIFT-MS can detect a range of VCs from PA in vitro. HCN may be a specific indicator of PA infection in vivo, and offers promise as a biomarker for noninvasive detection of PA infection by breath analysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
8755-6863
pubmed:author
pubmed:copyrightInfo
Copyright 2005 Wiley-Liss, Inc
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
452-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15765542-Acetonitriles, pubmed-meshheading:15765542-Adolescent, pubmed-meshheading:15765542-Adult, pubmed-meshheading:15765542-Ammonia, pubmed-meshheading:15765542-Biological Markers, pubmed-meshheading:15765542-Child, pubmed-meshheading:15765542-Child, Preschool, pubmed-meshheading:15765542-Cough, pubmed-meshheading:15765542-Culture Media, pubmed-meshheading:15765542-Cystic Fibrosis, pubmed-meshheading:15765542-Disulfides, pubmed-meshheading:15765542-Feasibility Studies, pubmed-meshheading:15765542-Humans, pubmed-meshheading:15765542-Hydrogen Cyanide, pubmed-meshheading:15765542-Mass Spectrometry, pubmed-meshheading:15765542-Odors, pubmed-meshheading:15765542-Pseudomonas Infections, pubmed-meshheading:15765542-Pseudomonas aeruginosa, pubmed-meshheading:15765542-Sensitivity and Specificity, pubmed-meshheading:15765542-Sputum, pubmed-meshheading:15765542-Sulfhydryl Compounds
pubmed:year
2005
pubmed:articleTitle
Detection of volatile compounds emitted by Pseudomonas aeruginosa using selected ion flow tube mass spectrometry.
pubmed:affiliation
Academic Department of Paediatrics, University Hospital of North Staffordshire, Stoke-on-Trent, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't