rdf:type |
|
lifeskim:mentions |
umls-concept:C0026022,
umls-concept:C0036849,
umls-concept:C0180860,
umls-concept:C0205171,
umls-concept:C0302523,
umls-concept:C0581406,
umls-concept:C0700325,
umls-concept:C0969686,
umls-concept:C1442518,
umls-concept:C1522664,
umls-concept:C1546637,
umls-concept:C1550638,
umls-concept:C1552652,
umls-concept:C1552685,
umls-concept:C1704449,
umls-concept:C1704684,
umls-concept:C1705195
|
pubmed:issue |
1
|
pubmed:dateCreated |
2005-3-14
|
pubmed:abstractText |
We constructed the fusion of peroxisomal acyl-CoA oxidase 3 and the enhanced yellow fluorescent protein (EYFP) for fluorescent labeling of Yarrowia lipolytica peroxisomes. Using the spectral overlap between EYFP and FM4-64, we developed a procedure for simultaneous observation of Y. lipolytica peroxisomes and vacuoles with the single fluorescein isothiocyanate filter set. Using this procedure we were able to follow the Y. lipolytica peroxisome-vacuole dynamics under pexophagy conditions and show that Y. lipolytica peroxisomes are degraded in the vacuoles by a macropexophagic mechanism.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acyl-CoA Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ethylamines,
http://linkedlifedata.com/resource/pubmed/chemical/FM 4-64,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oleic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridinium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Quaternary Ammonium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/yellow fluorescent protein, Bacteria
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
1065-6995
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
29
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
65-70
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:15763501-Acyl-CoA Oxidase,
pubmed-meshheading:15763501-Bacterial Proteins,
pubmed-meshheading:15763501-Ethylamines,
pubmed-meshheading:15763501-Fluorescent Dyes,
pubmed-meshheading:15763501-Luminescent Proteins,
pubmed-meshheading:15763501-Microscopy, Fluorescence,
pubmed-meshheading:15763501-Oleic Acid,
pubmed-meshheading:15763501-Peroxisomes,
pubmed-meshheading:15763501-Pyridinium Compounds,
pubmed-meshheading:15763501-Quaternary Ammonium Compounds,
pubmed-meshheading:15763501-Recombinant Fusion Proteins,
pubmed-meshheading:15763501-Vacuoles,
pubmed-meshheading:15763501-Yarrowia
|
pubmed:year |
2005
|
pubmed:articleTitle |
Observation of the Yarrowia lipolytica peroxisome-vacuole dynamics by fluorescence microscopy with a single filter set.
|
pubmed:affiliation |
Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street, 14/16, 79005 Lviv, Ukraine.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|