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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-3-14
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pubmed:abstractText |
We used a knock-in strategy to generate two lines of mice expressing Cre recombinase under the transcriptional control of the dopamine transporter promoter (DAT-cre mice) or the serotonin transporter promoter (SERT-cre mice). In DAT-cre mice, immunocytochemical staining of adult brains for the dopamine-synthetic enzyme tyrosine hydroxylase and for Cre recombinase revealed that virtually all dopaminergic neurons in the ventral midbrain expressed Cre. Crossing DAT-cre mice with ROSA26-stop-lacZ or ROSA26-stop-YFP reporter mice revealed a near perfect correlation between staining for tyrosine hydroxylase and beta-galactosidase or YFP. YFP-labeled fluorescent dopaminergic neurons could be readily identified in live slices. Crossing SERT-cre mice with the ROSA26-stop-lacZ or ROSA26-stop-YFP reporter mice similarly revealed a near perfect correlation between staining for serotonin-synthetic enzyme tryptophan hydroxylase and beta-galactosidase or YFP. Additional Cre expression in the thalamus and cortex was observed, reflecting the known pattern of transient SERT expression during early postnatal development. These findings suggest a general strategy of using neurotransmitter transporter promoters to drive selective Cre expression and thus control mutations in specific neurotransmitter systems. Crossed with fluorescent-gene reporters, this strategy tags neurons by neurotransmitter status, providing new tools for electrophysiology and imaging.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cre recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Plasma Membrane Transport...,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Plasma Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Slc6a3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Slc6a4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0165-0270
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27-32
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15763133-Animals,
pubmed-meshheading:15763133-Brain,
pubmed-meshheading:15763133-Chimera,
pubmed-meshheading:15763133-Dopamine,
pubmed-meshheading:15763133-Dopamine Plasma Membrane Transport Proteins,
pubmed-meshheading:15763133-Female,
pubmed-meshheading:15763133-Gene Expression Regulation,
pubmed-meshheading:15763133-Gene Targeting,
pubmed-meshheading:15763133-Genes, Reporter,
pubmed-meshheading:15763133-Integrases,
pubmed-meshheading:15763133-Male,
pubmed-meshheading:15763133-Membrane Glycoproteins,
pubmed-meshheading:15763133-Membrane Transport Proteins,
pubmed-meshheading:15763133-Mice,
pubmed-meshheading:15763133-Mice, Inbred C57BL,
pubmed-meshheading:15763133-Mice, Transgenic,
pubmed-meshheading:15763133-Molecular Biology,
pubmed-meshheading:15763133-Nerve Tissue Proteins,
pubmed-meshheading:15763133-Neurochemistry,
pubmed-meshheading:15763133-Neurons,
pubmed-meshheading:15763133-Promoter Regions, Genetic,
pubmed-meshheading:15763133-Recombinant Fusion Proteins,
pubmed-meshheading:15763133-Serotonin,
pubmed-meshheading:15763133-Serotonin Plasma Membrane Transport Proteins,
pubmed-meshheading:15763133-Tyrosine 3-Monooxygenase,
pubmed-meshheading:15763133-beta-Galactosidase
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pubmed:year |
2005
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pubmed:articleTitle |
Targeted gene expression in dopamine and serotonin neurons of the mouse brain.
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pubmed:affiliation |
Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, 924 East 57th Street, Knapp Center, R214, Chicago, IL 60637, USA. xzhuang@bsd.uchicago.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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