Linked Life Data

15756563

Source:http://linkedlifedata.com/resource/pubmed/id/15756563

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-3-9
pubmed:abstractText
The high mortality in patients with congenital diaphragmatic hernia (CDH) has been attributed to pulmonary hypoplasia and persistent pulmonary hypertension (PPH). Endothelin-1 (ET-1), nitric oxide (NO), and calcitonin gene-related peptide (CGRP) have been reported to be important vasoactive mediators in the perinatal pulmonary circulation. The exact mechanism by which these vasoactive mediators interact to regulate the perinatal pulmonary vascular tone in CDH with PPH is not fully understood. We hypothesized that the altered pulmonary vascular reactivity in CDH is due to imbalance in vasoactive mediators. This study was designed to investigate mRNA expression of ET-1, eNOS, and CGRP in CDH lung in the perinatal period. A CDH model was induced in pregnant rats following administration of nitrofen. In control animals, the same dose of olive oil was given without nitrofen. Cesarean section was performed on day 21 of gestation. The newborn rats were intubated and ventilated, and ventilation was continued for 1-6 h. Left lungs were collected from both groups at 0, 1, and 6 h after ventilation (n=8 in each group). Reverse transcriptase-polymerase chain reaction on lung tissue was performed to evaluate the relative level of ET-1, eNOS, and CGRP mRNA expression. The results showed a significant increase in ET-1 mRNA in CDH lung at 1 and 6 h after ventilation compared with controls. In CDH lung, eNOS mRNA and CGRP mRNA levels were significantly increased at 1 h but were similar to control values at 6 h after ventilation. The increased expression of vasoconstrictor ET-1 mRNA and vasodilators eNOS mRNA and CGRP mRNA in the CDH lung at 1 h after ventilation suggests that pulmonary vascular tone is rapidly changing after birth. An imbalance in the production of vasoconstrictors and vasodilators by the CDH lung may contribute to high pulmonary vascular resistance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0179-0358
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
143-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15756563-Animals, pubmed-meshheading:15756563-Animals, Newborn, pubmed-meshheading:15756563-Calcitonin Gene-Related Peptide, pubmed-meshheading:15756563-Disease Models, Animal, pubmed-meshheading:15756563-Endothelin-1, pubmed-meshheading:15756563-Female, pubmed-meshheading:15756563-Gene Expression Regulation, Developmental, pubmed-meshheading:15756563-Herbicides, pubmed-meshheading:15756563-Hernia, Diaphragmatic, pubmed-meshheading:15756563-Lung, pubmed-meshheading:15756563-Maternal Exposure, pubmed-meshheading:15756563-Nitric Oxide Synthase, pubmed-meshheading:15756563-Nitric Oxide Synthase Type III, pubmed-meshheading:15756563-Phenyl Ethers, pubmed-meshheading:15756563-Pregnancy, pubmed-meshheading:15756563-Prenatal Exposure Delayed Effects, pubmed-meshheading:15756563-Pulmonary Circulation, pubmed-meshheading:15756563-RNA, Messenger, pubmed-meshheading:15756563-Random Allocation, pubmed-meshheading:15756563-Rats, pubmed-meshheading:15756563-Rats, Sprague-Dawley, pubmed-meshheading:15756563-Respiration, Artificial, pubmed-meshheading:15756563-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15756563-Vascular Resistance, pubmed-meshheading:15756563-Vasoconstriction, pubmed-meshheading:15756563-Vasodilation
pubmed:year
2005
pubmed:articleTitle
Expression of vasoactive mediators during mechanical ventilation in nitrofen-induced diaphragmatic hernia in rats.
pubmed:affiliation
Children's Research Centre, Our Lady's Hospital for Sick Children, Dublin, Ireland.
pubmed:publicationType
Journal Article, Comparative Study