15750092

Source:http://linkedlifedata.com/resource/pubmed/id/15750092

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0004034, umls-concept:C0034754, umls-concept:C0040223, umls-concept:C0086045, umls-concept:C0439536, umls-concept:C0456389, umls-concept:C0936012, umls-concept:C1510438, umls-concept:C1697858
pubmed:issue	3
pubmed:dateCreated	2005-3-7
pubmed:abstractText	The influence of several test variables on susceptibility testing of Aspergillus spp. was assessed. A collection of 28 clinical isolates was tested against amphotericin B, itraconazole, voriconazole, and terbinafine. Inoculum size (10(4) CFU/ml versus 10(5) CFU/ml) and glucose supplementation (0.2% versus 2%) did not have significant effects on antifungal susceptibility testing results and higher inoculum size and glucose concentration did not falsely elevate MICs. In addition, antifungal susceptibility testing procedure with an inoculum size of 10(5) CFU/ml distinctly differentiated amphotericin B or itraconazole-resistant Aspergillus strains in vivo from the susceptible ones. Time of incubation significantly affected the final values of MICs, showing major increases (two to six twofold dilutions, P < 0.01 by analysis of variance) between MIC readings at 24 and 48 h, but no differences were observed between antifungal susceptibility testing results obtained at 48 h and at 72 h. Significantly higher MICs were uniformly associated with higher concentrations of Tween (P < 0.01), used as a dispersing agent in the preparation of inoculum suspensions. The geometric mean MICs showed increases of between 1.5- and 10-fold when the Tween concentration varied from 0.1% (the geometric means for amphotericin B, itraconazole, voriconazole, and terbinafine were 1.29, 0.69, 1.06, and 0.64 mug/ml, respectively) to 5% (the geometric means for amphotericin B, itraconazole, voriconazole, and terbinafine were 1.97, 5.79, 1.60, and 4.66 mug/ml, respectively). The inhibitory effect of Tween was clearly increased with inoculum sizes of 10(5) CFU/ml and was particularly dramatic for itraconazole, terbinafine, and Aspergillus terreus. The inoculum effect was not observed when the Tween concentration was below 0.5% (P > 0.01).
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-10194462, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-10196749, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-10348757, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-11158093, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-11158763, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-11283054, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-11302810, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-11481285, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-12121921, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-14605171, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-2007647, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-7559186, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-7615745, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-7730232, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-8722837, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-8980776, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-9087494, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-9174200, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-9210674, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-9338494, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-9559799, http://linkedlifedata.com/resource/pubmed/commentcorrection/15750092-9854079
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7505564
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, http://linkedlifedata.com/resource/pubmed/chemical/Polysorbates
pubmed:status	MEDLINE
pubmed:month	Mar
pubmed:issn	0095-1137
pubmed:author	pubmed-author:AberkaneAmelA, pubmed-author:Cuenca-EstrellaManuelM, pubmed-author:Gomez-LopezAliciaA, pubmed-author:MelladoEmiliaE, pubmed-author:PetrikkouEvaE, pubmed-author:Rodriguez-TudelaJuan LuisJL
pubmed:issnType	Print
pubmed:volume	43
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1251-5
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:15750092-Aspergillus, pubmed-meshheading:15750092-Culture Media, pubmed-meshheading:15750092-Dose-Response Relationship, Drug, pubmed-meshheading:15750092-Microbial Sensitivity Tests, pubmed-meshheading:15750092-Polysorbates, pubmed-meshheading:15750092-Time Factors
pubmed:year	2005
pubmed:articleTitle	Analysis of the influence of Tween concentration, inoculum size, assay medium, and reading time on susceptibility testing of Aspergillus spp.
pubmed:affiliation	Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo, Km 2. 28220 Majadahonda, Madrid, Spain.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't