Linked Life Data

15748828

Source:http://linkedlifedata.com/resource/pubmed/id/15748828

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-3-7
pubmed:abstractText
Our study focused on aromatase cytochrome P450 (CYP19) expression in ovarian epithelial normal and cancer cells and tissues. Aromatase mRNA expression was analyzed by real-time PCR in ovarian epithelial cancer cell lines, in human ovarian surface epithelial (HOSE) cell primary cultures, and in ovarian tissue specimens (n=94), including normal ovaries, ovarian cysts and cancers. Aromatase mRNA was found to be expressed in HOSE cells, in BG1, PEO4 and PEO14, but not in SKOV3 and NIH:OVCAR-3 ovarian cancer cell lines. Correlation analysis of aromatase expression was performed according to clinical, histological and biological parameters. Aromatase expression in ovarian tissue specimens was higher in normal ovaries and cysts than in cancers (P<0.0001). Using laser capture microdissection in normal postmenopausal ovaries, aromatase was found to be predominantly expressed in epithelial cells as compared to stromal component. Using immunohistochemistry (IHC), aromatase was also detected in the epithelium component. There was an inverse correlation between aromatase and ERalpha expression in ovarian tissues (P<0.001, r=-0.34). In the cancer group, no significant differences in aromatase expression were observed according to tumor histotype, grade, stage and survival. Aromatase activity was evaluated in ovarian epithelial cancer (OEC) cell lines by the tritiated water assay and the effects of third-generation aromatase inhibitors (AIs) on aromatase activity and growth were studied. Letrozole and exemestane were able to completely inhibit aromatase activity in BG1 and PEO14 cell lines. Interestingly, both AI showed an antiproliferative effect on the estrogen responsive BG1 cell line co-expressing aromatase and ERalpha. Aromatase expression was found in ovarian epithelial normal tissues and in some ovarian epithelial cancer cells and tissues. This finding raises the possibility that some tumors may respond to estrogen and provides a basis for ascertaining an antimitogenic effect of AI in a subgroup of ovarian epithelial cancers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0960-0760
pubmed:author
pubmed:issnType
Print
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15748828-Androstadienes, pubmed-meshheading:15748828-Aromatase, pubmed-meshheading:15748828-Aromatase Inhibitors, pubmed-meshheading:15748828-Cell Proliferation, pubmed-meshheading:15748828-Cysts, pubmed-meshheading:15748828-Epithelial Cells, pubmed-meshheading:15748828-Estrogen Receptor alpha, pubmed-meshheading:15748828-Female, pubmed-meshheading:15748828-Gene Expression Regulation, Enzymologic, pubmed-meshheading:15748828-Gene Expression Regulation, Neoplastic, pubmed-meshheading:15748828-Humans, pubmed-meshheading:15748828-Immunohistochemistry, pubmed-meshheading:15748828-Lasers, pubmed-meshheading:15748828-Microdissection, pubmed-meshheading:15748828-Middle Aged, pubmed-meshheading:15748828-Neoplasms, Glandular and Epithelial, pubmed-meshheading:15748828-Nitriles, pubmed-meshheading:15748828-Ovarian Neoplasms, pubmed-meshheading:15748828-Ovary, pubmed-meshheading:15748828-Polymerase Chain Reaction, pubmed-meshheading:15748828-RNA, Messenger, pubmed-meshheading:15748828-Triazoles, pubmed-meshheading:15748828-Tumor Cells, Cultured
pubmed:year
2005
pubmed:articleTitle
Aromatase expression in ovarian epithelial cancers.
pubmed:affiliation
Laboratoire de Biologie Cellulaire et Hormonale, Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5, France. s-cunat@chu-montpellier.fr
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't