Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1977-6-11
pubmed:abstractText
In this report, the properties of eight cases of amylase-linked immunoglobulins were gel filtration using Sephadex G-200 superfine. The class of heavy chain of amylase-linked immunoglobulins was proved to be gamma in four cases and alpha in three cases by immunoelectrophoresis followed by amylase activity staining. In one of the cases, the precipitin line against light chain lambda could be visualized only after treatment with 0.1 M 2-mercaptoethanol. Then, the type of light chain was determined to be exclusively lambda irrespective of heavy chain classes. In two cases, the immunoglobulin complexes were partially dissociated into normal amylase and immunoglobulin G at pH 8.6, and completely dissociated at pH 9.0. In three cases, the complexes were completely dissociated at pH 8.6. The precipitin line of papain treated amylase-linked immunoglobulin G against anti-Fc was not. This fact suggests that the binding site of amylase-linked immunoglobulins G was located in the Fab portion of immunoglobulin molecule and that the complexes are specific antigen-antibody complexes. Treatment with Con-A Sepharose caused the dissociation of the amylase-immunoglobulin complexes. It is suggested that the changes in the conformation of amylase-linked immunoglobulin causes the dissociation of this immuno-complex. Thus, it is elucidated that the complexes of amylase and immunoglobulins are specific antigen-antibody complexes, and that these complexes must be recognized as one of the circulating autoantibodies in plasma, and must be clearly distinguished from the other unknown macromolecular amylase complexes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0009-8981
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-77
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Properties of amylase-linked immunoglobulins.
pubmed:publicationType
Journal Article