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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
2005-5-23
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pubmed:abstractText |
Ultraviolet light-induced apoptosis can be caused by DNA damage but also involves immediate-early cell death cascades characteristic of death receptor signaling. Here we show that the UV light-induced apoptotic signaling pathway is unique, targeting Bax activation at the mitochondrial membrane independent of caspase-8 or cathepsin D activity. Cells deficient in acid sphingomyelinase (ASMase) do not show UV light-induced Bax activation, cytochrome c release, or apoptosis. In ASMase-deficient cells, the apoptotic UV light response is restored by stable or transient expression of human ASMase. Bax conformational change in ASMase(-/-) cells is also caused by synthetic C(16)-ceramide acting on intact cells or isolated mitochondria. The results suggest that UV light-triggered ASMase activation is essentially required for Bax conformational change leading to mitochondrial release of pro-apoptotic factors like cytochrome c and Smac.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CASP8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 8,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin D,
http://linkedlifedata.com/resource/pubmed/chemical/Ceramides,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingomyelin Phosphodiesterase,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
280
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20804-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15743760-Apoptosis,
pubmed-meshheading:15743760-Caspase 3,
pubmed-meshheading:15743760-Caspase 8,
pubmed-meshheading:15743760-Caspases,
pubmed-meshheading:15743760-Cathepsin D,
pubmed-meshheading:15743760-Cell Membrane,
pubmed-meshheading:15743760-Cell-Free System,
pubmed-meshheading:15743760-Ceramides,
pubmed-meshheading:15743760-Cytochromes c,
pubmed-meshheading:15743760-Fluorescent Antibody Technique,
pubmed-meshheading:15743760-Gene Expression,
pubmed-meshheading:15743760-Green Fluorescent Proteins,
pubmed-meshheading:15743760-HeLa Cells,
pubmed-meshheading:15743760-Humans,
pubmed-meshheading:15743760-Mitochondria,
pubmed-meshheading:15743760-Niemann-Pick Diseases,
pubmed-meshheading:15743760-Protein Conformation,
pubmed-meshheading:15743760-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15743760-RNA, Small Interfering,
pubmed-meshheading:15743760-Signal Transduction,
pubmed-meshheading:15743760-Sphingomyelin Phosphodiesterase,
pubmed-meshheading:15743760-Ultraviolet Rays,
pubmed-meshheading:15743760-bcl-2-Associated X Protein
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pubmed:year |
2005
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pubmed:articleTitle |
Acid sphingomyelinase is indispensable for UV light-induced Bax conformational change at the mitochondrial membrane.
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pubmed:affiliation |
Institute for Medical Microbiology, Immunology and Hygiene and Center for Molecular Medicine Cologne, University of Cologne, 50935 Cologne, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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