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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1977-6-22
|
| pubmed:abstractText |
We describe the preparation of a new substrate, N-gamma-L-glutamyl-5-aminoisophthalic acid dimethyl ester hydro-chloride, for the fluorometric determination of gamma-glutamyltransferase activity in serum by the "front-surface" technique. Details of the resulting method are provided. The final reaction mixture contains 4 mmol of the substrate per liter of tris(hydroxymethyl)aminomethane (100 mmol/liter) and glycylglycine (75 mmol/liter) at pH 8.2 (25 degrees C).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0009-9147
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
879-81
|
| pubmed:dateRevised |
2000-12-18
|
| pubmed:meshHeading |
pubmed-meshheading:15741-Buffers,
pubmed-meshheading:15741-Colorimetry,
pubmed-meshheading:15741-Fluorometry,
pubmed-meshheading:15741-Hydrogen-Ion Concentration,
pubmed-meshheading:15741-Methods,
pubmed-meshheading:15741-Phthalic Acids,
pubmed-meshheading:15741-Quinine,
pubmed-meshheading:15741-Spectrometry, Fluorescence,
pubmed-meshheading:15741-gamma-Glutamyltransferase
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
New substrate for fluorometric determination of gamma-glutamyltransferase activity in serum.
|
| pubmed:publicationType |
Journal Article
|