| pubmed-article:15740899 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15740899 | lifeskim:mentions | umls-concept:C0006901 | lld:lifeskim |
| pubmed-article:15740899 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:15740899 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:15740899 | lifeskim:mentions | umls-concept:C0436196 | lld:lifeskim |
| pubmed-article:15740899 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:15740899 | lifeskim:mentions | umls-concept:C0392762 | lld:lifeskim |
| pubmed-article:15740899 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15740899 | pubmed:dateCreated | 2005-3-2 | lld:pubmed |
| pubmed-article:15740899 | pubmed:abstractText | On-line capillary isoelectric focusing-mass spectrometry (cIEF-MS) was applied to determine concentrations of peptides and proteins using angiotensin II and human tetrasialo-transferrin as the model samples. The concentration of the carrier ampholyte was optimized for both resolution and ion intensity. cIEF-MS employing 1% Pharmalyte 3-10 and a sheath liquid containing water/methanol/acetic acid (50/49/1) resolved angiotensin I and II (5 microM each, DeltapI=0.2) at an Rs value of 2.29. The determined concentration of angiotensin II (0.1-5 microM) well correlated (R=0.999) with that obtained by the conventional RP-HPLC method. The limit of detection was 0.22 microM, which was about 10 times lower than that by UV detection (2 microM). The repeatability and accuracy were <15 and <11%, respectively. cIEF-MS was also applied to determine human tetrasialo-transferrin concentration. The good linearity (R2=0.998) was also observed between the transferrin concentration (0.5-1.2 g/L) and peak area ratio (IS; beta-lactoglobulin B) with acceptable accuracy (<1.9%) and repeatability ( approximately 10% at 1g/L). | lld:pubmed |
| pubmed-article:15740899 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15740899 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15740899 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15740899 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15740899 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15740899 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15740899 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15740899 | pubmed:issn | 0731-7085 | lld:pubmed |
| pubmed-article:15740899 | pubmed:author | pubmed-author:ShibukawaAkim... | lld:pubmed |
| pubmed-article:15740899 | pubmed:author | pubmed-author:KurodaYukihir... | lld:pubmed |
| pubmed-article:15740899 | pubmed:author | pubmed-author:NakagawaTerum... | lld:pubmed |
| pubmed-article:15740899 | pubmed:author | pubmed-author:MatsuzakiKats... | lld:pubmed |
| pubmed-article:15740899 | pubmed:author | pubmed-author:KitanoMasahir... | lld:pubmed |
| pubmed-article:15740899 | pubmed:author | pubmed-author:YukinagaHideo... | lld:pubmed |
| pubmed-article:15740899 | pubmed:author | pubmed-author:NoguchiTaekoT | lld:pubmed |
| pubmed-article:15740899 | pubmed:author | pubmed-author:NematiMahboob... | lld:pubmed |
| pubmed-article:15740899 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15740899 | pubmed:day | 9 | lld:pubmed |
| pubmed-article:15740899 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:15740899 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15740899 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15740899 | pubmed:pagination | 423-8 | lld:pubmed |
| pubmed-article:15740899 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:meshHeading | pubmed-meshheading:15740899... | lld:pubmed |
| pubmed-article:15740899 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15740899 | pubmed:articleTitle | On-line capillary isoelectric focusing-mass spectrometry for quantitative analysis of peptides and proteins. | lld:pubmed |
| pubmed-article:15740899 | pubmed:affiliation | Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan. y.kuroda@scas.co.jp | lld:pubmed |
| pubmed-article:15740899 | pubmed:publicationType | Journal Article | lld:pubmed |
