Source:http://linkedlifedata.com/resource/pubmed/id/15740899
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2005-3-2
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pubmed:abstractText |
On-line capillary isoelectric focusing-mass spectrometry (cIEF-MS) was applied to determine concentrations of peptides and proteins using angiotensin II and human tetrasialo-transferrin as the model samples. The concentration of the carrier ampholyte was optimized for both resolution and ion intensity. cIEF-MS employing 1% Pharmalyte 3-10 and a sheath liquid containing water/methanol/acetic acid (50/49/1) resolved angiotensin I and II (5 microM each, DeltapI=0.2) at an Rs value of 2.29. The determined concentration of angiotensin II (0.1-5 microM) well correlated (R=0.999) with that obtained by the conventional RP-HPLC method. The limit of detection was 0.22 microM, which was about 10 times lower than that by UV detection (2 microM). The repeatability and accuracy were <15 and <11%, respectively. cIEF-MS was also applied to determine human tetrasialo-transferrin concentration. The good linearity (R2=0.998) was also observed between the transferrin concentration (0.5-1.2 g/L) and peak area ratio (IS; beta-lactoglobulin B) with acceptable accuracy (<1.9%) and repeatability ( approximately 10% at 1g/L).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0731-7085
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
423-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15740899-Animals,
pubmed-meshheading:15740899-Cattle,
pubmed-meshheading:15740899-Electrophoresis, Capillary,
pubmed-meshheading:15740899-Humans,
pubmed-meshheading:15740899-Isoelectric Focusing,
pubmed-meshheading:15740899-Mass Spectrometry,
pubmed-meshheading:15740899-Online Systems,
pubmed-meshheading:15740899-Peptides,
pubmed-meshheading:15740899-Proteins,
pubmed-meshheading:15740899-Sheep
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pubmed:year |
2005
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pubmed:articleTitle |
On-line capillary isoelectric focusing-mass spectrometry for quantitative analysis of peptides and proteins.
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pubmed:affiliation |
Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan. y.kuroda@scas.co.jp
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pubmed:publicationType |
Journal Article
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