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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2005-3-2
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pubmed:abstractText |
Adult murine epidermis contains members of the epithelial gammadelta T-cell family called dendritic epidermal T cells (DETCs). Their development and maturation have been the subjects of investigations, but the details are still unclear. T-cell receptor (TCR) zeta-chain-associated protein-70 (ZAP-70), one of the protein tyrosine kinases required for TCR signaling, plays a pivotal role in the development of alphabeta T cells. In mice lacking ZAP-70, thymic development of alphabeta T cells was completely arrested at the immature CD4(+)CD8(+) TCR(low) stage. Here, we examined whether or not the development and maturation of DETCs were altered in ZAP-70-deficient mice. Immunohistochemical analyses of epidermal sheets revealed that the number of DETCs was reduced and their characteristic dendrites were lost or markedly shortened in ZAP-70(-/-) mice. In flow cytometric analyses, the expression levels of gammadelta TCR and Thy-1.2 on the ZAP-70(-/-) DETCs were lower than those on ZAP-70(+/-) DETCs. The expression of a very early activation antigen, CD69, was not detected on ZAP-70(-/-) DETCs, whereas CD69 was expressed on ZAP-70(+/-) DETCs. Furthermore, ZAP-70(-/-) DETCs showed markedly reduced proliferation and no IL-2 gene expression in response to anti-CD3epsilon or concanavalin A stimulation. These results suggest that ZAP-70 is essential for TCR signaling which induces the proliferation and the final maturation of DETCs in the epidermis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD69 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Cd3e protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Thy28 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/ZAP-70 Protein-Tyrosine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Zap70 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0906-6705
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
188-93
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15740591-Animals,
pubmed-meshheading:15740591-Antibodies, Monoclonal,
pubmed-meshheading:15740591-Antigens, CD,
pubmed-meshheading:15740591-Antigens, CD3,
pubmed-meshheading:15740591-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:15740591-Cell Aging,
pubmed-meshheading:15740591-Cell Count,
pubmed-meshheading:15740591-Cell Proliferation,
pubmed-meshheading:15740591-Cells, Cultured,
pubmed-meshheading:15740591-Concanavalin A,
pubmed-meshheading:15740591-Dendritic Cells,
pubmed-meshheading:15740591-Epidermis,
pubmed-meshheading:15740591-Gene Expression,
pubmed-meshheading:15740591-Interleukin-2,
pubmed-meshheading:15740591-Lectins, C-Type,
pubmed-meshheading:15740591-Mice,
pubmed-meshheading:15740591-Mice, Knockout,
pubmed-meshheading:15740591-Nuclear Proteins,
pubmed-meshheading:15740591-Phenotype,
pubmed-meshheading:15740591-Protein-Tyrosine Kinases,
pubmed-meshheading:15740591-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:15740591-T-Lymphocytes,
pubmed-meshheading:15740591-ZAP-70 Protein-Tyrosine Kinase
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pubmed:year |
2005
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pubmed:articleTitle |
Zeta-chain-associated protein-70 molecule is essential for the proliferation and the final maturation of dendritic epidermal T cells.
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pubmed:affiliation |
Department of Dermatology, Gunma University Graduate School of Medicine, 3-39-22 Showa machi, Maebashi Gunma 371-8511, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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