Linked Life Data

15739474

Source:http://linkedlifedata.com/resource/pubmed/id/15739474

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-3-2
pubmed:abstractText
A sensitive method of high performance liquid chromatography (HPLC) for determination of liensinine in plasma is reported. An Ultrasphere Si column, 250 mm x 4.6 mm i.d. with dichloromethaneisopropyl alcohol-diethylamine (75:25:0.2, V/V) as mobile phase at a flow-rate of 1.0 mL/min and UV-detector at 282 nm were used. Neferine was served as the internal standard. Liensinine in plasma was extracted with diethyl ether three times after adding ammonia-ammonium chloride buffer (pH 10). Liensinine was completely separated from nefrine. The retention times of liensinine and neferine were 8.5 and 5.0 minutes, respectively. The calibration line of liensinine was linear in the range of 0.0625-5.0 mg/L (r = 0.9996). The mean recovery was 93.6% with RSD of 1.9%. The precision of intra-day and inter-day for liensinine was in the range of 1.4%-4.1% and the detectable limit was 0.025 mg/L based on a signal-to-noise ratio of 3. The results showed that the method was simple and sensitive.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1000-8713
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
347-8
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
[Determination of liensinine in plasma by high performance liquid chromatography].
pubmed:affiliation
Department of Pharmacy, Hubei Medical University, Wuhan, 430060.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't, Evaluation Studies