Linked Life Data

15739324

Source:http://linkedlifedata.com/resource/pubmed/id/15739324

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-3-2
pubmed:abstractText
Perilla oil was verified to be a rich source of polyunsaturated fatty acid (PUFA). Its linolenic content is 64.82%, the highest in the plants. Its triacylglycerol (TAG) components were isolated and identified first time in this paper by a combination of non-aqueous reversed-phase high performance liquid chromatography (RP-HPLC) with gas chromatography (GC). The TAG of perilla oil were isolated by HPLC with a Zorbax ODS column (5 microm, 4.6 mm i.d. x 250 mm; Dupont, Inc) and differential refractometer. The eluent was acetone/acetonitrile (80:20, V/V) with flow rate of 1.0 mL/min. The TAG component acyl groups were converted to fatty acid methyl esters (FAME). The acyl constituents for each TAG were determined by GC analysis. Gas chromatography of FAMEs was performed with a AC20 Carbowax 20M column (30 m x 0.32 mm i.d.). Detector and injection port temperatures were 260 degrees C. Column temperature was programmed from 120 degrees C, 1 min initially hold, then 8 degrees C/min to 220 degrees C and finally hold for 10 min. Nitrogen was the carrier gas. FAMEs peaks were identified by comparison of their retention times with those of standards. The retention times of the palmitic, stearic, oleic, linoleic, n-nonadecanoic acid (internal standard) and linolenic FAMEs were 10.7, 13.0, 13.3, 13.8, 14.2 and 14.7 min respectively. Five main components were determined. They were LnLnLn (34.10%), LnLnL (13.22%), LnLL (6.22%), LnLnO (11.38%) and LnLnP (9.17%). The TAG contents were corrected from their fatty acids content. The combination of non-aqueous RP-HPLC with GC is a simple and rapid method for the analysis of oil TAG structure and is also very helpful for the identification of oil quality.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1000-8713
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
470-3
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
[Analysis of triacylglycerol composition of perilla oil by combination of non-aqueous reversed-phase high performance liquid chromatography with gas chromatography].
pubmed:affiliation
Wuxi University of Light Industry, Wuxi, 214036.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't, Evaluation Studies